Prüss B M
Department of Microbiology and Immunology, University of Illinois at Chicago, IL 60612-7344, USA.
Arch Microbiol. 1998 Sep;170(3):141-6. doi: 10.1007/s002030050626.
Carbon sources that can be converted to acetate were added to the growth medium of Escherichia coli wild-type cells. Cells responded with an increased cell division rate. The addition of acetate also caused a decreased synthesis of flagella. Mutants in phosphotransacetylase, which are incapable of synthesizing acetyl phosphate, and mutants in the osmoregulator OmpR divided at a lower rate than did wild-type cells. The mutants did not increase their cell division rate upon the addition of serine, as observed for wild-type cells. These data are consistent with the idea that the previously described effect of serine upon the cell division rate is mediated by acetyl phosphate and phosphorylation of OmpR.
可转化为乙酸盐的碳源被添加到大肠杆菌野生型细胞的生长培养基中。细胞的反应是细胞分裂速率增加。乙酸盐的添加还导致鞭毛合成减少。磷酸转乙酰酶突变体无法合成乙酰磷酸，渗透压调节蛋白OmpR的突变体分裂速率低于野生型细胞。与野生型细胞不同，这些突变体在添加丝氨酸后细胞分裂速率并未增加。这些数据与以下观点一致，即先前描述的丝氨酸对细胞分裂速率的影响是由乙酰磷酸和OmpR的磷酸化介导的。
