Pettinari M J, Vazquez G J, Krüger N, Vary P S, Steinbüchel A, Méndez B S
Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Appl Microbiol Biotechnol. 1998 Jun;49(6):737-42. doi: 10.1007/s002530051240.
A Bacillus megaterium genomic fragment, which encoded an activator homologous to sigma 54 regulators and which was capable of activating Escherichia coli ato genes in trans, was detected in a gene library of B. megaterium screened for beta-ketothiolase activity. The fragment presented only one complete open reading frame (ORF1), which encoded a protein of 398 amino acids. The recombinant plasmid complemented mutations in the Escherichia coli atoC regulatory gene. The constitutive expression of the E. coli ato operon mediated by ORF1 could be useful for the synthesis of polyhydroxyalkanoates with different flexibility properties by recombinant E. coli strains.
在巨大芽孢杆菌基因文库中筛选β-酮硫解酶活性时，检测到一个巨大芽孢杆菌基因组片段，该片段编码一种与σ54调节因子同源的激活剂，能够反式激活大肠杆菌的ato基因。该片段仅呈现一个完整的开放阅读框（ORF1），其编码一个含398个氨基酸的蛋白质。重组质粒弥补了大肠杆菌atoC调节基因中的突变。由ORF1介导的大肠杆菌ato操纵子的组成型表达，可能有助于重组大肠杆菌菌株合成具有不同柔韧性特性的聚羟基脂肪酸酯。
