Kaetsu H, Mizuguchi J, Hamamoto T, Kamimura K, Yoshida Y, Nakagaki T, Ogata Y, Miyamoto S, Funatsu A
The Chemo-Sero-Therapeutic Research Institute, Kumamoto, Okubo, Japan.
Thromb Res. 1998 May 1;90(3):101-9. doi: 10.1016/s0049-3848(98)00026-7.
We investigated the ability of polyethylene glycol 4000 to accelerate thrombin generation in a mixture of prothrombin and factor X at concentrations of 1-30%. In the presence of 5 mM of CaCl2, polyethylene glycol 4000 promoted prothrombin activation at concentrations above 1%. The peak of activation was seen at levels of 14 and 20% of polyethylene glycol 4000. The effect of the polyethylene glycol was remarkably dependent on its molecular weight; molecular weights greater than 2000 were required for accelerating thrombin generation. Under optimal conditions, polyethylene glycol 4000, in the presence of CaCl2, promoted conversion of all of the prothrombin into thrombin and its derivatives. We conclude that polyethylene glycol 4000, at concentrations ranging from 14 to 20%, effectively accelerates thrombin generation in the presence of 5 mM of CaCl2. This new method for preparing thrombin is based on the use of polyethylene glycol 4000 and CaCl2 and is applicable to the manufacture of thrombin.
我们研究了聚乙二醇4000在浓度为1%至30%的凝血酶原和因子X混合物中加速凝血酶生成的能力。在存在5 mM氯化钙的情况下，聚乙二醇4000在浓度高于1%时促进凝血酶原激活。在聚乙二醇4000浓度为14%和20%时观察到激活峰值。聚乙二醇的作用显著依赖于其分子量；加速凝血酶生成需要分子量大于2000。在最佳条件下，聚乙二醇4000在氯化钙存在下促进所有凝血酶原转化为凝血酶及其衍生物。我们得出结论，在存在5 mM氯化钙的情况下，浓度为14%至20%的聚乙二醇4000能有效加速凝血酶生成。这种制备凝血酶的新方法基于聚乙二醇4000和氯化钙的使用，适用于凝血酶的制造。
