CD4受体的细胞外结构域具有蛋白激酶活性。

The extracellular domain of CD4 receptor possesses a protein kinase activity.

作者信息

Yakubov L A, Kit Y Y, Richter V A, Andreeva A Y, Karamyshev V N, Stein C A, Vlassov V V

机构信息

Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences.

出版信息

FEBS Lett. 1998 Jul 10;431(1):45-8. doi: 10.1016/s0014-5793(98)00721-2.

DOI:10.1016/s0014-5793(98)00721-2

PMID:9684862

Abstract

The CD4 receptor of T-helper cells is an essential participant in immune response formation and HIV infection. We report here that the extracellular domains of CD4 receptor can catalyze the phosphotransferase (kinase) reaction. Incubation of rsCD4 in solution with [gamma-32P]ATP results in the Ca2+-dependent autophosphorylation of the protein presumably at a His residue because the reaction is prevented by the diethylpyrocarbonate treatment. The rsCD4 phosphorylates milk casein or human plasma proteins as a Ser/Thr protein kinase.

摘要

辅助性T细胞的CD4受体是免疫应答形成和HIV感染中的重要参与者。我们在此报告，CD4受体的胞外结构域可催化磷酸转移酶（激酶）反应。将重组可溶性CD4（rsCD4）与[γ-32P]ATP在溶液中孵育，会导致该蛋白可能在组氨酸残基处发生Ca2+依赖性自磷酸化，因为焦碳酸二乙酯处理可阻止该反应。rsCD4作为一种丝氨酸/苏氨酸蛋白激酶可使乳酪蛋白或人血浆蛋白磷酸化。