重组腺病毒介导的基因体内递送至大鼠视网膜色素上皮细胞。
Recombinant adenovirus-mediated gene delivery into the rat retinal pigment epithelium in vivo.
作者信息
Rakoczy P E, Lai C M, Shen W Y, Daw N, Constable I J
机构信息
Centre of Ophthalmology and Visual Science, The University of Western Australia, Nedlands, Australia.
出版信息
Aust N Z J Ophthalmol. 1998 May;26 Suppl 1:S56-8. doi: 10.1111/j.1442-9071.1998.tb01373.x.
PURPOSE
The present paper describes changes following the subretinal injection of a recombinant replication-deficient adenovirus carrying the beta-galactosidase reporter gene construct into the rat retina.
METHODS
Ad.RSV.betagal-mediated transduction of rat retinal pigment epithelium (RPE) cells in vitro and in vivo was examined following X-gal staining.
RESULTS
There was a low level of beta-galactosidase expression in the RPE cells at 4 days postinjection. At 7 days postinjection, a strong transgene expression was present in RPE cells and the expression was maintained at 14 days postinjection. Except for the accumulation of cells at the site of the injection, the morphology of the rest of the retina remained normal.
CONCLUSION
These results demonstrate that the RPE layer can be successfully targeted for gene delivery in the rat.
目的
本文描述了将携带β-半乳糖苷酶报告基因构建体的重组复制缺陷型腺病毒视网膜下注射到大鼠视网膜后所发生的变化。
方法
在进行X-gal染色后，检测Ad.RSV.betagal介导的大鼠视网膜色素上皮（RPE）细胞在体外和体内的转导情况。
结果
注射后4天，RPE细胞中β-半乳糖苷酶表达水平较低。注射后7天，RPE细胞中出现强烈的转基因表达，且在注射后14天仍维持该表达。除注射部位细胞聚集外，视网膜其余部分形态保持正常。
结论
这些结果表明，在大鼠中可成功将RPE层作为基因递送的靶点。
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