冷冻保存后人视网膜动脉血管活性特性的保留
Preservation of vasoactive properties of human retinal arteries after cryopreservation.
作者信息
Su E N, Yu D Y, Cringle S, Alder V, Yu P, Buckland L
机构信息
Lions Eye Institute, The University of Western Australia, Nedlands, Australia.
出版信息
Aust N Z J Ophthalmol. 1998 May;26 Suppl 1:S59-61. doi: 10.1111/j.1442-9071.1998.tb01374.x.
PURPOSE
Although we have several established in vitro systems for studying the vasoactive properties of the ocular vasculature, the restricted availability of human donor tissue has forced us to rely predominantly on animal studies.
METHODS
The present paper describes, for the first time, a controlled-rate cryopreservation technique capable of maintaining vasoactive function in human retinal arteries after extended storage.
RESULTS/CONCLUSION: The response to constrictive (endothelin-I) and vasodilatory agents (nimodipine and betaxolol) was comparable with that in fresh pig vessels. It is now possible to obtain multiple segments of retinal artery from a single donor eye and to preserve them for detailed pharmacological assessment.
目的
尽管我们拥有多个用于研究眼部血管系统血管活性特性的体外系统，但人类供体组织的有限可用性迫使我们主要依赖动物研究。
方法
本文首次描述了一种控速冷冻保存技术，该技术能够在长期保存后维持人视网膜动脉的血管活性功能。
结果/结论：对收缩剂（内皮素-I）和血管扩张剂（尼莫地平和倍他洛尔）的反应与新鲜猪血管中的反应相当。现在可以从单只供体眼中获取多个视网膜动脉节段，并将它们保存起来用于详细的药理学评估。
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