腺相关病毒介导的基因转移至人视网膜色素上皮细胞。
Adeno-associated virus-mediated gene transfer into human retinal pigment epithelium cells.
作者信息
Lai Y K, Rakoczy P, Constable I, Rolling F
机构信息
Centre for Ophthalmology and Visual Science, University of Western Australia, Perth, Australia.
出版信息
Aust N Z J Ophthalmol. 1998 May;26 Suppl 1:S77-9. doi: 10.1111/j.1442-9071.1998.tb01381.x.
PURPOSE
Adeno-associated virus (AAV) is emerging as a promising vector for gene therapy
METHOD
To determine the ability of recombinant AAV (rAAV) to express and integrate exogenous DNA into human retinal pigment epithelium (RPE) cells, a rAAV-GFP vector containing the green fluorescent protein (gfp) and neomycin resistance (neo(r)) genes was constructed and used to transduce RPE 407A cell line.
RESULTS
Fluorescent RPE cell clones were obtained and were confirmed to still be expressing GFP after 24 passages (3.5 months).
CONCLUSION
Adeno-associated virus-based vectors are able to efficiently transduce and stably persist in RPE cells.
目的
腺相关病毒（AAV）正成为一种有前景的基因治疗载体。
方法
为了确定重组腺相关病毒（rAAV）表达外源DNA并将其整合到人类视网膜色素上皮（RPE）细胞中的能力，构建了一种包含绿色荧光蛋白（gfp）和新霉素抗性（neo(r)）基因的rAAV-GFP载体，并用于转导RPE 407A细胞系。
结果
获得了荧光RPE细胞克隆，并证实其在传代24次（3.5个月）后仍在表达GFP。
结论
基于腺相关病毒的载体能够有效地转导并稳定存在于RPE细胞中。
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