Lu B, Leygue E, Dotzlaw H, Murphy L J, Murphy L C, Watson P H
Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.
Mol Cell Endocrinol. 1998 Mar 16;138(1-2):199-203. doi: 10.1016/s0303-7207(98)00050-1.
Estrogen receptor (ER)-beta mRNA splice variants have been identified in human breast tumors as well as normal human and mouse ovarian, uterine and mammary tissues. In both species transcripts deleted in exons 5 or 6, or 5 + 6 have been characterized by RT-PCR followed by cloning and sequencing. In mouse tissues an ER-beta transcript containing 54 nucleotides inserted in frame between exons 5 and 6 was identified. Interestingly, no equivalent of the mouse inserted transcript was detected in any of the four human tissues analyzed.
雌激素受体（ER）-β信使核糖核酸剪接变体已在人类乳腺肿瘤以及正常人类和小鼠的卵巢、子宫和乳腺组织中被鉴定出来。在这两个物种中，通过逆转录聚合酶链反应（RT-PCR），随后进行克隆和测序，对在外显子5或6或5 + 6中缺失的转录本进行了表征。在小鼠组织中，鉴定出一种在5号和6号外显子之间框内插入54个核苷酸的ER-β转录本。有趣的是，在所分析的四个人类组织中均未检测到与小鼠插入转录本等效的转录本。
