通过体内足迹法对丝状真菌中蛋白质 - DNA 相互作用进行原位检测。
In situ detection of protein-DNA interactions in filamentous fungi by in vivo footprinting.
作者信息
Wolschek M F, Narendja F, Karlseder J, Kubicek C P, Scazzocchio C, Strauss J
机构信息
IBTM, Technical University Vienna, Getreidemarkt 9/172-5, A-1060 Vienna, Austria, Institut für Biochemie,Vienna Biocenter, Dr. Bohr Gasse 9, A-1030 Vienna, Austria.
出版信息
Nucleic Acids Res. 1998 Aug 15;26(16):3862-4. doi: 10.1093/nar/26.16.3862.
Abstract
The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform modification of all cells in an apically growing, non-uniform organism, and subsequent visualization of protected areas by ligation-mediated PCR.
摘要
此处所述方法可用于检测丝状真菌体内的蛋白质 - DNA 相互作用。我们概述了培养条件和体内甲基化条件，这些条件能使顶端生长、结构不均一的生物体中的所有细胞实现均匀修饰，随后通过连接介导的 PCR 对受保护区域进行可视化。
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