Grab D J, Givens C, Kennedy R
Department of Parasitology, Tulane Regional Primate Research Center, Covington, LA, USA.
Biochim Biophys Acta. 1998 Aug 14;1407(2):135-45. doi: 10.1016/s0925-4439(98)00038-6.
Recently, the term MSCRAMM (microbial surface components recognizing adhesive matrix molecules), has been introduced to describe microbial molecules that recognize extracellular matrix (ECM) [1]. Here we present evidence for the presence of fibronectin-binding molecules in Borrelia burgdorferi and several other Borrelia species. Immunofluorescence studies show that plasma fibronectin is bound uniformly over the cell surface of free swimming B. burgdorferi. In addition, the spirochetes are able to bind to plasma fibronectin-coated microwell plates, an interaction that is inhibited by anti-fibronectin antibody as well as exogenous plasma fibronectin. Taken together, the data suggest that fibronectin binds to the surface of the spirochete. On Western blot-like assays, B. burgdorferi and some B. afzelii strains express a major fibronectin-binding protein (Fn-BA) with an approximate molecular mass of 52 kDa. In addition, several other major Fn-BAs were found in B. hermsii (26, 31, 33, 39, 46, 54 and 58 kDa) and B. turicatae (39, 41, 45, 50, 56, 59 and 66 kDa). Preliminary evidence suggests that fibronectin (and Fn-BA) may play a role as a molecular bridge between the spirochete and other components of the extracellular matrix.
最近，引入了“微生物表面成分识别黏附基质分子”（MSCRAMM）这一术语来描述识别细胞外基质（ECM）的微生物分子[1]。在此，我们提供证据表明伯氏疏螺旋体及其他几种疏螺旋体物种中存在纤连蛋白结合分子。免疫荧光研究显示，血浆纤连蛋白均匀地结合在自由游动的伯氏疏螺旋体细胞表面。此外，螺旋体能够结合到包被有血浆纤连蛋白的微孔板上，这种相互作用可被抗纤连蛋白抗体以及外源性血浆纤连蛋白抑制。综合这些数据表明，纤连蛋白结合到螺旋体表面。在类似蛋白质印迹的分析中，伯氏疏螺旋体和一些阿氏疏螺旋体菌株表达一种主要的纤连蛋白结合蛋白（Fn - BA），其近似分子量为52 kDa。此外，在赫氏疏螺旋体（26、31、33、39、46、54和58 kDa）和杜氏疏螺旋体（39、41、45、50、56、59和66 kDa）中还发现了其他几种主要的Fn - BA。初步证据表明，纤连蛋白（和Fn - BA）可能作为螺旋体与细胞外基质其他成分之间的分子桥梁发挥作用。
