可溶性鸟苷酸环化酶α亚基在已鉴定昆虫神经元中的定位

Localization of soluble guanylyl cyclase alpha-subunit in identified insect neurons.

作者信息

Elphick M R, Jones I W

机构信息

School of Biological Sciences, Queen Mary and Westfield College, University of London, Mile End Road, London E1 4NS, UK.

出版信息

Brain Res. 1998 Jul 27;800(1):174-9. doi: 10.1016/s0006-8993(98)00522-8.

DOI:10.1016/s0006-8993(98)00522-8

PMID:9685632

Abstract

The distribution of soluble guanylyl cyclase in the brain of the locust Schistocerca gregaria was analysed using antisera to a highly conserved region (X-peptide) of the Drosophila soluble guanylyl cyclase alpha-subunit (SGCalpha). Analysis of locust brain and locust eye homogenates in Western blots using X-peptide antisera revealed specific staining of a approximately 65 kDa band, which is similar to the expected molecular mass for a SGCalpha-subunit. SGCalpha-immunoreactivity was localized in identified neuronal components of several sensory systems including photoreceptors of the compound eyes and ocelli, large ocellar interneurons, antennal mechanosensory neurons and olfactory interneurons. These neurons are putative targets for the gas nitric oxide which activates guanylyl cyclase activity in the locust brain.

摘要

利用针对果蝇可溶性鸟苷酸环化酶α亚基（SGCalpha）高度保守区域（X肽）的抗血清，分析了沙漠蝗大脑中可溶性鸟苷酸环化酶的分布。使用X肽抗血清对蝗虫大脑和蝗虫眼睛匀浆进行蛋白质免疫印迹分析，结果显示一条约65 kDa条带出现特异性染色，这与SGCalpha亚基预期的分子量相似。SGCalpha免疫反应性定位于几个感觉系统中已确定的神经元成分，包括复眼和单眼的光感受器、大的单眼中间神经元、触角机械感觉神经元和嗅觉中间神经元。这些神经元可能是气体一氧化氮的作用靶点，一氧化氮可激活蝗虫大脑中的鸟苷酸环化酶活性。