[用于确认HIV-1抗体的蛋白质印迹系统（DAVIH-BLOT）的评估]
[Evaluation of a western blot system (DAVIH-BLOT) for the confirmation of HIV-1 antibodies].
作者信息
Cruz Sui O, Pérez Guevara M T, Izquierdo Márquez M, Lobaina Batelemy L, Ruibal Brunet I, Silva Cabrera E
机构信息
Centro de Investigaciones Científicas, Defensa Civil Laboratorio de Investigaciones del Sida.
出版信息
Rev Cubana Med Trop. 1997;49(1):28-31.
It is reported the evaluation of the DAVIH-BLOT system (DAVIH Laboratories, Cuba) against the proficiency panels sent to the National AIDS Reference Laboratory by the World Health Organization (WHO) in order to ensure the quality in the diagnosis of HIV antibodies during 1989, 1991 and 1993. The system is also compared with those of the Dupont and Diagnostic Pasteur firms in a group of 467 positive sera by ELISA. On studying the WHO's samples, the system proved to be highly sensitive and specific. In the comparison made with the HIV-1 Western Blot IgG version 1.2 (Dupont company, USA) and with the LAV-blot 1 (Diagnostic Pasteur, France), there was a coincidence of 100 and 97%, respectively.
据报道，对DAVIH - BLOT系统（古巴DAVIH实验室）针对世界卫生组织（WHO）发送给国家艾滋病参比实验室的能力验证样本进行了评估，以确保1989年、1991年和1993年期间HIV抗体诊断的质量。该系统还通过酶联免疫吸附测定法（ELISA）与杜邦公司和巴斯德诊断公司的系统在一组467份阳性血清中进行了比较。在研究WHO的样本时，该系统被证明具有高度的敏感性和特异性。在与HIV - 1免疫印迹IgG版本1.2（美国杜邦公司）和LAV - blot 1（法国巴斯德诊断公司）进行的比较中，符合率分别为100%和97%。
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