Mendiola J, Casanova P, Hernández H, Fernández-Calienes A, García I
Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba.
Rev Cubana Med Trop. 1997;49(3):161-6.
In the present paper it was determined the concentration of immunoglobulin G in extracts from gut of female Boophilus microplus engorded during the first 7 days of the second stage of continual digestion by using the simple radial immunodiffusion technique. It was also measured the concentration of hemoglobin by spectrophotometric methods. The analysis of correlation between both variables made during the study was positive and significant for p < 0.05. The analysis of simple variance and the application of Duncan's test resulted in a marked reduction of the concentrations of hemoglobin in the gut from the 5th day of study, which is connected with the beginning of the active process of oviposition, and in an important decrease of the concentration of immunoglobulin from the 7th day of the study. Though there is a trend to diminish in the concentrations of both blood proteins, the proteolysis of the IgG by gut extracts could not be produced in vitro.
在本论文中，采用简单的放射免疫扩散技术测定了在连续消化第二阶段的前7天育肥的微小牛蜱雌虫肠道提取物中免疫球蛋白G的浓度。还通过分光光度法测定了血红蛋白的浓度。在研究过程中对这两个变量进行的相关性分析呈正相关，且在p < 0.05时具有显著性。单因素方差分析和邓肯检验的应用结果表明，从研究的第5天开始，肠道中血红蛋白的浓度显著降低，这与产卵活跃过程的开始有关，并且从研究的第7天开始免疫球蛋白的浓度大幅下降。尽管两种血液蛋白的浓度都有下降趋势，但肠道提取物在体外不能使IgG发生蛋白水解。
