Espino A M, Padrón L, Duménigo B, Laferté J
Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba.
Rev Cubana Med Trop. 1997;49(3):167-73.
The standardization of an ultramicroELISA for the detection of IgG antibodies anti excretory-secretory antigens of Fasciola hepatica (UME-Fasciola) is described. It was studied a considerable group of sera of which 56 were from patients with fascioliasis, 168 from patients with other parasitic diseases, and 300 from sound persons that were used as negative controls. As regards the parasitology test considered as "Gold Standard", the UME-Fasciola showed a sensitivity of 100%, an specificity of 98%, and predictive values for positives and negatives of 90.3% and 100%, respectively. Cross-reaction was only observed with the sera from patients infected with Opistorchis felineus. On comparing the UME-Fasciola with the conventional ELISA, it was obtained a concordance index of 95.5%.
本文描述了一种用于检测抗肝片吸虫排泄-分泌抗原IgG抗体的超微量酶联免疫吸附测定法（UME-Fasciola）的标准化过程。研究了相当数量的血清样本，其中56份来自肝片吸虫病患者，168份来自其他寄生虫病患者，300份来自健康人作为阴性对照。就被视为“金标准”的寄生虫学检测而言，UME-Fasciola的灵敏度为100%，特异性为98%，阳性预测值和阴性预测值分别为90.3%和100%。仅在感染猫后睾吸虫患者的血清中观察到交叉反应。将UME-Fasciola与传统酶联免疫吸附测定法进行比较，一致性指数为95.5%。
