Bibollet-Ruche F, Loussert-Ajaka I, Simon F, Mboup S, Ngole E M, Saman E, Delaporte E, Peeters M
Project SIDAK, Laboratoire Rétrovirus, ORSTOM, Montpellier, France.
AIDS Res Hum Retroviruses. 1998 Jul 20;14(11):951-61. doi: 10.1089/aid.1998.14.951.
Human immunodeficiency virus type 1 (HIV-1) group O strains have been described as highly divergent, compared with the vast majority of the viruses involved in the worldwide AIDS pandemic, classified in group M. To gain new insights into the diversity and genetic characteristics of group O, we have sequenced the accessory gene region (from vif to vpu) of 14 isolates. Analyses of the deduced amino acid sequences for Vif, Vpr, the first exon of Tat, and Vpu indicate that most of the functional domains of these proteins, as described for group M viruses, are highly conserved and retained among all the group O strains we have characterized. The only difference concerns the Vif phosphorylation sites, which are absent in all of the group O isolates we have sequenced; in contrast, they are well conserved in nearly all of the group M isolates, in which they play critical roles in the regulation of viral replication and infectivity. As already observed for group M isolates, the Vpu protein is also highly diverse among group O strains. Phylogenetic analyses of these sequences indicate that HIV-1 group O can be separated into four different clusters, containing most of the strains we have characterized (except one, which clusters outside of the analyzed viruses). Taking into account the criteria used for clades in group M, we were not able to define group O clades definitively.
与全球艾滋病大流行中绝大多数归为M组的病毒相比，1型人类免疫缺陷病毒（HIV-1）O组毒株被描述为具有高度的差异性。为了深入了解O组的多样性和基因特征，我们对14株病毒的辅助基因区域（从vif到vpu）进行了测序。对Vif、Vpr、Tat的第一个外显子以及Vpu推导的氨基酸序列分析表明，正如M组病毒中所描述的那样，这些蛋白质的大多数功能域在我们所鉴定的所有O组毒株中高度保守且得以保留。唯一的差异在于Vif磷酸化位点，在我们测序的所有O组毒株中均不存在；相反，它们在几乎所有M组毒株中都高度保守，在这些毒株中它们在病毒复制和感染性的调控中发挥关键作用。正如在M组毒株中已经观察到的那样，Vpu蛋白在O组毒株中也高度多样。对这些序列的系统发育分析表明，HIV-1 O组可分为四个不同的簇，包含了我们所鉴定的大多数毒株（除了一个在分析的病毒之外聚类的毒株）。考虑到M组中用于分支的标准，我们无法明确界定O组的分支。
