牛甲状腺素结合球蛋白。与人类甲状腺素结合球蛋白的分子量及氨基酸组成的纯化与比较。
Bovine thyroxine-binding globulin. Purification and comparison of molecular weight and amino acid composition with human thyroxine-binding globulin.
作者信息
van der Walt B J, van Jaarsveld P P
出版信息
Biochim Biophys Acta. 1978 Jul 21;535(1):44-53. doi: 10.1016/0005-2795(78)90031-4.
Bovine and human thyroxine-binding globulin were purified from serum by a three-step purification procedure which comprised affinity chromatography consecutively on thyroxine- and Concanavalin A--Sepharose and finally preparative polyacrylamide gel electrophoresis. The molecular weights of the two proteins were similar (54 000) as well as their carbohydrate contents while some differences in amino acid composition were found. Rabbit antiserum against bovine thyroxine-binding globulin reacted with human thyroxine-binding globulin with no sign of spur formation.
通过三步纯化程序从血清中纯化牛和人甲状腺素结合球蛋白，该程序包括依次在甲状腺素和伴刀豆球蛋白A-琼脂糖上进行亲和层析，最后进行制备性聚丙烯酰胺凝胶电泳。两种蛋白质的分子量相似（54000），碳水化合物含量也相似，但氨基酸组成存在一些差异。抗牛甲状腺素结合球蛋白的兔抗血清与人甲状腺素结合球蛋白发生反应，没有形成刺的迹象。
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