Beckmann A, Gebhardt F, Brandt B H
Institute of Clinical Chemistry and Laboratory Medicine, Muenster, Germany.
J Chromatogr B Biomed Sci Appl. 1998 Jun 12;710(1-2):75-80. doi: 10.1016/s0378-4347(98)00135-2.
To assess gene dosages for clinical application, especially for prognostication of cancer, we developed a direct quantification method for polymerase chain reaction products. We report on an application of field amplified sample injection (FASI) to capillary zone electrophoresis which allows the quantification of PCR products without sample preparation. Using an external standard and UV detection for the quantification of DNA, a low coefficient of variation has been obtained. Overall, the described method provides a fast and easy tool for PCR product quantification in clinical laboratories.
为评估基因剂量在临床应用中的情况，尤其是在癌症预后方面，我们开发了一种用于聚合酶链反应产物的直接定量方法。我们报告了场放大进样（FASI）在毛细管区带电泳中的应用，该方法可在无需样品制备的情况下对PCR产物进行定量。通过使用外标和紫外检测对DNA进行定量，获得了较低的变异系数。总体而言，所描述的方法为临床实验室中PCR产物的定量提供了一种快速且简便的工具。
