Hounsom L, Horrobin D F, Tritschler H, Corder R, Tomlinson D R
Department of Pharmacology, Queen Mary and Westfield College, London, UK.
Diabetologia. 1998 Jul;41(7):839-43. doi: 10.1007/s001250050996.
Untreated streptozotocin-diabetic (7 weeks duration) rats showed reductions (all p < 0.01; percentages in brackets) in motor and sensory nerve conduction velocity (MNCV; 14%, SNCV; 17%) and in sciatic nerve contents of nerve growth factor (NGF; 57%), substance P (SP; 53%) and neuropeptide Y (NPY; 39%). Treatment with a gamma-linolenic acid-alpha-lipoic acid conjugate (GLA-LA; 35 mg x day(-1) x rat(-1)) attenuated (p < 0.05) these reductions to MNCV (8%), SNCV (5%), NGF (19%), SP (23%), NPY (20%), such that the values in GLA-LA-treated diabetic rats did not differ significantly from those of control non-diabetic animals. Treatment with alpha-lipoic acid alone at 100 mg/kg i.p. was without effect on these variables except for NGF (33% reduction, p < 0.05) and treatment with the antioxidant, butylated hydroxytoluene (1.5% dietary supplement) did not affect any deficits. These data show that GLA-LA is effective in improving both electrophysiological and neurochemical correlates of experimental diabetic neuropathy.
未经治疗的链脲佐菌素诱导的糖尿病大鼠（病程7周）显示运动和感觉神经传导速度（运动神经传导速度降低14%，感觉神经传导速度降低17%）以及坐骨神经中神经生长因子（降低57%）、P物质（降低53%）和神经肽Y（降低39%）含量均下降（所有p<0.01；括号内为百分比）。用γ-亚麻酸-α-硫辛酸共轭物（GLA-LA；35mg·天⁻¹·大鼠⁻¹）治疗可减轻（p<0.05）这些指标的下降，运动神经传导速度下降至8%，感觉神经传导速度下降至5%，神经生长因子下降至19%，P物质下降至23%，神经肽Y下降至20%，使得接受GLA-LA治疗的糖尿病大鼠的这些值与对照非糖尿病动物的值无显著差异。单独腹腔注射100mg/kg的α-硫辛酸对这些变量无影响，但对神经生长因子有影响（降低33%，p<0.05），而抗氧化剂丁基羟基甲苯（1.5%膳食补充剂）治疗对任何缺陷均无影响。这些数据表明，GLA-LA可有效改善实验性糖尿病神经病变的电生理和神经化学相关指标。
