Fricchione G L, Bilfinger T V, Stefano G B
Division of Psychiatry, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Int J Cardiol. 1998 Apr 30;64 Suppl 1:S29-33. doi: 10.1016/s0167-5273(98)00033-3.
We examined the effects of aspirin on human immunocyte adherence to human saphenous vein preparations in vitro. Monocytes and granulocytes were analyzed by phase-contrast microscopy in conjunction with a time-lapse video recording system for cell counting in the medium. Saphenous vein samples taken from five volunteer subjects during their elective coronary artery bypass grafting procedures were used for an in vitro experiment in which each subject's immune cells were added to his/her respective endothelial tissues. In addition, aspirin or morphine was added to the preparations. After 30 min the endothelium and the medium were examined for cell numbers using feature color detection software. The endothelium exposed to a 10 min preincubation with 10 mmol/l aspirin was found to have significantly fewer monocytes and granulocytes attached. The saphenous vein preparations were examined for nitric oxide (NO) release using an NO-selective amperometric microprobe. Aspirin at its immunocyte inhibitory concentrations did not induce NO release from the endothelium, whereas morphine, which is known to stimulate NO production by endothelium, did so. These findings demonstrate that the administration of aspirin in high doses can diminish human granulocyte and monocyte adherence to saphenous vein endothelium in vitro through an NO-independent mechanism.
我们在体外研究了阿司匹林对人免疫细胞黏附于人大隐静脉制剂的影响。通过相差显微镜结合延时视频记录系统对培养基中的单核细胞和粒细胞进行分析以进行细胞计数。从五名志愿者在择期冠状动脉旁路移植手术过程中采集的大隐静脉样本用于体外实验，其中将每个受试者的免疫细胞添加到其各自的内皮组织中。此外，将阿司匹林或吗啡添加到制剂中。30分钟后，使用特征颜色检测软件检查内皮和培养基中的细胞数量。发现用10 mmol/L阿司匹林预孵育10分钟的内皮上附着的单核细胞和粒细胞明显减少。使用NO选择性电流微探针检测大隐静脉制剂中的一氧化氮（NO）释放。处于免疫细胞抑制浓度的阿司匹林不会诱导内皮释放NO，而众所周知能刺激内皮产生NO的吗啡则会诱导内皮释放NO。这些发现表明，高剂量阿司匹林给药可通过不依赖NO的机制减少体外人粒细胞和单核细胞对大隐静脉内皮的黏附。
