通过荧光原位杂交技术评估细菌细胞壁的状态。
Estimation of the state of the bacterial cell wall by fluorescent In situ hybridization.
作者信息
Bidnenko E, Mercier C, Tremblay J, Tailliez P, Kulakauskas S
机构信息
Génétique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas, France.
出版信息
Appl Environ Microbiol. 1998 Aug;64(8):3059-62. doi: 10.1128/AEM.64.8.3059-3062.1998.
Abstract
Fluorescent in situ hybridization (FISH) is now a widely used method for identification of bacteria at the single-cell level. With gram-positive bacteria, the thick peptidoglycan layer of a cell wall presents a barrier for entry of horseradish peroxidase (HRP)-labeled probes. Therefore, such probes do not give any signal in FISH unless cells are first treated with enzymes which hydrolyze the peptidoglycan. We explored this feature of FISH to detect cells which have undergone permeabilization due to expression of autolytic enzymes. Our results indicate that FISH performed with HRP-labeled probes provides a sensitive method to estimate the states of cell walls of individual gram-positive bacteria.
摘要
荧光原位杂交（FISH）现在是一种广泛用于在单细胞水平鉴定细菌的方法。对于革兰氏阳性菌，细胞壁厚厚的肽聚糖层对辣根过氧化物酶（HRP）标记探针的进入构成障碍。因此，除非细胞先用水解肽聚糖的酶处理，否则此类探针在FISH中不会产生任何信号。我们利用FISH的这一特性来检测因自溶酶表达而发生通透性改变的细胞。我们的结果表明，用HRP标记探针进行的FISH提供了一种敏感的方法来评估单个革兰氏阳性菌的细胞壁状态。
相似文献
1
Estimation of the state of the bacterial cell wall by fluorescent In situ hybridization.通过荧光原位杂交技术评估细菌细胞壁的状态。
Appl Environ Microbiol. 1998 Aug;64(8):3059-62. doi: 10.1128/AEM.64.8.3059-3062.1998.
2
16S rRNA-targeted oligonucleotide probes for direct detection of Propionibacterium freudenreichii in presence of Lactococcus lactis with multicolour fluorescence in situ hybridization.16S rRNA 靶向寡核苷酸探针用于多色荧光原位杂交直接检测乳球菌存在下的弗雷德氏丙酸杆菌。
Lett Appl Microbiol. 2014 Sep;59(3):320-7. doi: 10.1111/lam.12278. Epub 2014 Jun 2.
3
Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification.通过辣根过氧化物酶标记的寡核苷酸与酪胺信号放大相结合提高全细胞杂交的灵敏度。
Appl Environ Microbiol. 1997 Aug;63(8):3268-73. doi: 10.1128/aem.63.8.3268-3273.1997.
4
In situ identification of cyanobacteria with horseradish peroxidase-labeled, rRNA-targeted oligonucleotide probes.利用辣根过氧化物酶标记的、靶向rRNA的寡核苷酸探针进行蓝细菌的原位鉴定。
Appl Environ Microbiol. 1999 Mar;65(3):1259-67. doi: 10.1128/AEM.65.3.1259-1267.1999.
5
Differential sensitivity of 16S rRNA targeted oligonucleotide probes used for fluorescence in situ hybridization is a result of ribosomal higher order structure.用于荧光原位杂交的针对16S rRNA的寡核苷酸探针的差异敏感性是核糖体高级结构的结果。
Can J Microbiol. 1996 Oct;42(10):1061-71. doi: 10.1139/m96-136.
6
Fluorescence in situ hybridization for the identification of environmental microbes.用于鉴定环境微生物的荧光原位杂交技术。
Methods Mol Biol. 2007;353:153-64. doi: 10.1385/1-59745-229-7:153.
7
Autolytic phenotype of Lactococcus lactis strains isolated from traditional Tunisian dairy products.从突尼斯传统乳制品中分离出的乳酸乳球菌菌株的自溶表型。
J Appl Microbiol. 2002;92(5):812-20. doi: 10.1046/j.1365-2672.2002.01585.x.
8
Lactic acid bacteria as vaccine delivery vehicles.作为疫苗递送载体的乳酸菌。
Antonie Van Leeuwenhoek. 1996 Oct;70(2-4):317-30. doi: 10.1007/BF00395939.
9
Horseradish peroxidase-labeled oligonucleotides and fluorescent tyramides for rapid detection of chromosome-specific repeat sequences.用于快速检测染色体特异性重复序列的辣根过氧化物酶标记寡核苷酸和荧光酪胺
Cytogenet Cell Genet. 1996;75(4):258-62. doi: 10.1159/000134496.
10
Effects of a muramidase on a mixed bacterial community.一种溶菌酶对混合细菌群落的影响。
FEMS Microbiol Lett. 2000 Jun 1;187(1):47-52. doi: 10.1111/j.1574-6968.2000.tb09135.x.
引用本文的文献
1
Development of a fixation-free fluorescence hybridization for the detection of species.用于物种检测的免固定荧光杂交技术的开发。
Biol Methods Protoc. 2020 Dec 16;5(1):bpaa024. doi: 10.1093/biomethods/bpaa024. eCollection 2020.
2
Immobilization of Phosphatidylserine by Ethanol and Lysozyme on the Cell Surface for Evaluation of Apoptosis-Like Decay in Activated-Sludge Bacteria.利用乙醇和溶菌酶将磷脂酰丝氨酸固定在细胞表面，用于评估活性污泥细菌中类似凋亡的衰减。
Appl Environ Microbiol. 2020 Jul 2;86(14). doi: 10.1128/AEM.00345-20.
3
Genetics of Lactococci.乳球菌的遗传学。
Microbiol Spectr. 2019 Jul;7(4). doi: 10.1128/microbiolspec.GPP3-0035-2018.
4
Cell Walls and the Convergent Evolution of the Viral Envelope.细胞壁与病毒包膜的趋同进化
Microbiol Mol Biol Rev. 2015 Dec;79(4):403-18. doi: 10.1128/MMBR.00017-15.
5
Genome sequence and characterization of the Tsukamurella bacteriophage TPA2.基因组序列和特性分析Tsukamurella 噬菌体 TPA2。
Appl Environ Microbiol. 2011 Feb;77(4):1389-98. doi: 10.1128/AEM.01938-10. Epub 2010 Dec 23.
6
Detection of bacteriophage-infected cells of Lactococcus lactis by using flow cytometry.利用流式细胞术检测乳酸乳球菌的噬菌体感染细胞
Appl Environ Microbiol. 2007 Dec;73(23):7575-81. doi: 10.1128/AEM.01219-07. Epub 2007 Oct 5.
7
Potential interactions of particle-associated anammox bacteria with bacterial and archaeal partners in the Namibian upwelling system.纳米比亚上升流系统中与颗粒相关的厌氧氨氧化细菌与细菌和古菌伙伴之间的潜在相互作用。
Appl Environ Microbiol. 2007 Jul;73(14):4648-57. doi: 10.1128/AEM.02774-06. Epub 2007 May 25.
8
Microbial community structure and dynamics in the largest natural French lake (Lake Bourget).法国最大的天然湖泊（布尔歇湖）中的微生物群落结构与动态
Microb Ecol. 2006 Jul;52(1):72-89. doi: 10.1007/s00248-004-0230-4. Epub 2006 May 29.
9
Unlabeled helper oligonucleotides increase the in situ accessibility to 16S rRNA of fluorescently labeled oligonucleotide probes.未标记的辅助寡核苷酸增加了荧光标记寡核苷酸探针与16S rRNA的原位可及性。
Appl Environ Microbiol. 2000 Aug;66(8):3603-7. doi: 10.1128/AEM.66.8.3603-3607.2000.
10
Survival, physiology, and lysis of Lactococcus lactis in the digestive tract.乳酸乳球菌在消化道中的存活、生理学特性及裂解
Appl Environ Microbiol. 1999 Nov;65(11):4881-6. doi: 10.1128/AEM.65.11.4881-4886.1999.
本文引用的文献
1
Improved medium for lactic streptococci and their bacteriophages.用于乳酸链球菌及其噬菌体的改良培养基。
Appl Microbiol. 1975 Jun;29(6):807-13. doi: 10.1128/am.29.6.807-813.1975.
2
Comparative Study of 35 Bacteriophages of Lactobacillus helveticus: Morphology and Host Range.瑞士乳杆菌 35 株噬菌体的比较研究：形态和宿主范围。
Appl Environ Microbiol. 1992 Mar;58(3):1011-8. doi: 10.1128/aem.58.3.1011-1018.1992.
3
Analysis of the DNA sequence, gene expression, origin of replication and modular structure of the Lactococcus lactis lytic bacteriophage sk1.乳酸乳球菌裂解性噬菌体sk1的DNA序列、基因表达、复制起点及模块结构分析。
Mol Microbiol. 1997 Oct;26(1):49-64. doi: 10.1046/j.1365-2958.1997.5491926.x.
4
Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification.通过辣根过氧化物酶标记的寡核苷酸与酪胺信号放大相结合提高全细胞杂交的灵敏度。
Appl Environ Microbiol. 1997 Aug;63(8):3268-73. doi: 10.1128/aem.63.8.3268-3273.1997.
5
Sequencing and analysis of the prolate-headed lactococcal bacteriophage c2 genome and identification of the structural genes.长头乳球菌噬菌体c2基因组的测序与分析及结构基因的鉴定
Appl Environ Microbiol. 1995 Dec;61(12):4348-56. doi: 10.1128/aem.61.12.4348-4356.1995.
6
Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation.乳酸乳球菌主要肽聚糖水解酶（一种细胞分裂所需的溶菌酶）编码基因的分子克隆及核苷酸序列
J Bacteriol. 1995 Mar;177(6):1554-63. doi: 10.1128/jb.177.6.1554-1563.1995.
7
Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms.利用核糖体RNA荧光原位杂交技术测量年轻和成熟生物膜中单个细胞的活性。
Appl Environ Microbiol. 1993 May;59(5):1354-60. doi: 10.1128/aem.59.5.1354-1360.1993.
8
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.无需培养的单个微生物细胞的系统发育鉴定和原位检测。
Microbiol Rev. 1995 Mar;59(1):143-69. doi: 10.1128/mr.59.1.143-169.1995.
9
Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing.原生质体诱导消除后乳酸乳球菌NCDO 712及其他乳酸链球菌的质粒互补体
J Bacteriol. 1983 Apr;154(1):1-9. doi: 10.1128/jb.154.1.1-9.1983.
10
Structure, function, and assembly of cell walls of gram-positive bacteria.革兰氏阳性菌细胞壁的结构、功能及组装
Annu Rev Microbiol. 1983;37:501-27. doi: 10.1146/annurev.mi.37.100183.002441.
Zotero 插件