位点和链特异性DNA断裂赋予裂殖酵母不对称转换潜力。
A site- and strand-specific DNA break confers asymmetric switching potential in fission yeast.
作者信息
Arcangioli B
机构信息
Unité des Virus Oncogénes, URA 1644 du CNRS, Department des Biotechnologies, Institut Pasteur, Paris, France.
出版信息
EMBO J. 1998 Aug 3;17(15):4503-10. doi: 10.1093/emboj/17.15.4503.
Abstract
Mating-type switching in the fission yeast Schizosaccharomyces pombe results in the transfer of genetic information from one of the two silent cassettes (mat2P or mat3M) to the transcriptionally active locus (mat1). The switching pattern is programmed by an imprinting event which restricts mat1 gene conversion to only one of the two sister cells, leading to asymmetric cell division. Biochemical analysis indicated that the mat1 locus contains a fragile chromosomal site. Southern hybridization and primer extension experiments showed that the fragility consists of a single-strand break (SSB). The nicked DNA is stable throughout the cell cycle. The features of the nick fulfil all the requirements for the 'epigenetic', site and strand-specific chromosome modification at the mat1 locus, providing strong evidence that an SSB can initiate mitotic and meiotic gene conversion during replication.
摘要
裂殖酵母粟酒裂殖酵母中的交配型转换导致遗传信息从两个沉默盒（mat2P或mat3M）之一转移到转录活性位点（mat1）。转换模式由印记事件编程，该事件将mat1基因转换限制在两个姐妹细胞中的仅一个，导致不对称细胞分裂。生化分析表明，mat1位点包含一个脆弱的染色体位点。Southern杂交和引物延伸实验表明，脆弱性由单链断裂（SSB）组成。带切口的DNA在整个细胞周期中都是稳定的。切口的特征满足了mat1位点“表观遗传”、位点和链特异性染色体修饰的所有要求，有力地证明了SSB可以在复制过程中启动有丝分裂和减数分裂基因转换。
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