Holz RA, Wright DJ, Perry RN
Department of Biology, Imperial College at Silwood Park, Ascot, Berks, SL5 7PY, UK
J Helminthol. 1998 Jun;72(2):133-41. doi: 10.1017/s0022149x0001631x.
The lipid composition of three batches of single generation cysts of Globodera rostochiensis, stored dry at 4 degreesC for 1, 7 and 13 years, comprised 81%, 74% and 53% neutral lipids, 14%, 18% and 27% non-acidic phospholipids and 5%, 8% and 20% free fatty acids, respectively. Lipids in eggs from two batches of G. pallida cysts, stored for 3 and 7 years, comprised 80% and 67% neutral lipids, 15% and 23% non-acidic phospholipids and 5% and 10% free fatty acids, respectively. All batches contained the same fatty acids which were dominated by C18:1, C20:1 and C20:4. The fatty acid profiles of hatched J2 of G. rostochiensis from two batches, stored for 1 and 9 years, differed only in their free fatty acid fractions. Thus, while it is not possible to determine the age of cysts by their fatty acid profile, it may be possible to use the relative amounts of the main lipid classes as an indicator of age. Four batches of hatched J2 of G. pallida were investigated, with sample A hatched during the second week in potato root diffusate, B during week 3, C during week 4 and D during weeks 5 and 6 and stored for 3.5 days (on average) after hatching. Total lipid content was 27.2%, 31.5%, 18.5% and 6.3% of the dry weight for A, B, C and D, respectively. In the neutral lipid fraction of D an increase in C18:1 and to a lesser extent C18:2 was observed. In the free fatty acid fraction of sample D, the percentages of C18:1, C18:2 and C18:3 were greater but the percentages of C20:3 and C20:4 were smaller compared with sample C. Fresh early hatched J2 of G. rostochiensis were compared with later hatched and stored (for 13 days on average) individuals for their lipid content and fatty acid composition. The lipid content was 26.1% and 11.4% in fresh and stored J2, respectively. Total lipid consisted of 77% and 70% neutral lipid, 18% and 26% non-acidic phospholipid and 6% and 4% free fatty acid in fresh and stored J2, respectively. In the neutral lipid fraction of stored J2 C18:1, C16:0 and C18:0 increased, whereas C20:4, C20:1 and C20:3 decreased. Therefore, both neutral lipid and free fatty acid fractions showed changes in their fatty acid profiles after long delayed hatching and/or storage in both PCN species.
将罗氏茎线虫三代单胞囊在4℃干燥保存1年、7年和13年后，其脂质组成分别为中性脂质81%、74%和53%，非酸性磷脂14%、18%和27%，游离脂肪酸5%、8%和20%。将两批马铃薯白线虫胞囊保存3年和7年后的卵中脂质组成分别为中性脂质80%和67%，非酸性磷脂15%和23%，游离脂肪酸5%和10%。所有批次均含有相同的脂肪酸，以C18:1、C20:1和C20:4为主。两批分别保存1年和9年的罗氏茎线虫孵化J2的脂肪酸谱仅在游离脂肪酸部分有所不同。因此，虽然无法通过脂肪酸谱确定胞囊的年龄，但有可能将主要脂质类别的相对含量用作年龄指标。对四批马铃薯白线虫孵化J2进行了研究，样品A在马铃薯根浸出液中第二周孵化，B在第三周孵化，C在第四周孵化，D在第五周和第六周孵化，孵化后平均保存3.5天。A、B、C和D的总脂质含量分别为干重的27.2%、31.5%、18.5%和6.3%。在D的中性脂质部分，观察到C18:1增加，C18:2增加程度较小。与样品C相比，样品D的游离脂肪酸部分中C18:1、C18:2和C18:3的百分比更高，但C20:3和C20:4的百分比更低。对新鲜早期孵化的罗氏茎线虫J2与后期孵化并保存（平均13天）的个体的脂质含量和脂肪酸组成进行了比较。新鲜和保存的J2中脂质含量分别为26.1%和11.4%。新鲜和保存的J2中总脂质分别由77%和70%的中性脂质、18%和26%的非酸性磷脂以及6%和4%的游离脂肪酸组成。在保存的J2的中性脂质部分，C18:1、C16:0和C18:0增加，而C20:4、C20:1和C20:3减少。因此，在两种马铃薯胞囊线虫中，长时间延迟孵化和/或保存后，中性脂质和游离脂肪酸部分的脂肪酸谱均发生了变化。
