Neuburger M, Herget G W, Plaumann L, Falk A, Schwalb H, Adler C P
Department of Anaesthesiology and Intensive Care, KKH Bretten, Germany.
Pathol Res Pract. 1998;194(6):385-9. doi: 10.1016/s0344-0338(98)80028-9.
In the present study we examined light-microscopically the nuclei of 16 hearts (7 normal hearts, 7 hearts with hypertrophy and 2 hearts with atrophy) for the size, number and morphology of their nucleoli. The size of the nucleoli, defined as the total area of all nucleoli per nucleus, was an early and sensitive indication of a beginning hypertrophy of the myocardium. It increased in parallel to the total heart weight and to the total area of the nucleus of the cell, but initially the size of the nucleoli changed earlier than the other parameters. We found an increase of the ratio in the total area of the nucleoli per nucleus to the total area of the nucleus at the beginning of clinical hyperfunction. This ratio normalised during chronic hyperfunction. The number of the nucleoli also increased during hyperfunction, but it did not exceed the number of nucleolus organiser regions (NOR) given by the number of chromosomes. Nevertheless, we found numbers of nucleoli higher than 10 because, the number of NORs increases during polyploidization. Regarding the morphology of the nucleoli in hearts with hypertrophy, we found a predomination of the nucleoli with a highly branched nucleolonemal structure as an indication of an increased RNA synthesis.
在本研究中，我们通过光学显微镜检查了16颗心脏（7颗正常心脏、7颗肥大心脏和2颗萎缩心脏）的细胞核，以观察其核仁的大小、数量和形态。核仁大小定义为每个细胞核中所有核仁的总面积，是心肌开始肥大的早期敏感指标。它与心脏总重量和细胞核总面积平行增加，但最初核仁大小的变化比其他参数更早。我们发现在临床功能亢进开始时，每个细胞核中核仁总面积与细胞核总面积的比值增加。在慢性功能亢进期间，该比值恢复正常。在功能亢进期间，核仁数量也增加，但不超过由染色体数量给出的核仁组织区（NOR）数量。然而，我们发现核仁数量高于10，因为在多倍体化过程中NOR数量增加。关于肥大心脏中核仁的形态，我们发现具有高度分支的核仁丝结构的核仁占主导，这表明RNA合成增加。
