Madej A, Belowski D, Kowalski J, Herman Z S
Department of Clinical Pharmacology Silesian University School of Medicine, Katowice, Poland.
Boll Chim Farm. 1998 May;137(5):135-9.
The aim of the study was to evaluate the influence of neuroleptics on the in vivo and in vitro activities of rat spleen macrophages. In the in vivo study, three neuroleptics (chlorpromazine, haloperidol, and sulpiride) were given once, for 14 or 28 days. In the in vitro study, we evaluated the effects of two different concentrations of the neuroleptics on 3-day cultures of spleen macrophages. Rat spleen macrophages were isolated by the adherence method, and their cytotoxic activity was determined by measuring 51 Cr release from target cells P-815. In the in vitro study, both concentrations of all neuroleptics did not alter the cytotoxic activity of macrophages. In the in vivo study, neuroleptics (chlorpromazine 2 mg/kg, haloperidol 0.5 mg/kg, sulpiride 50 mg/kg) enhanced the cytotoxicity of macrophages both after a single injection and after 14 days. The results of the study indicate that the immunomodulatory effects of the neuroleptics depend mainly on dosage and experimental conditions.
本研究的目的是评估抗精神病药物对大鼠脾脏巨噬细胞体内和体外活性的影响。在体内研究中，三种抗精神病药物（氯丙嗪、氟哌啶醇和舒必利）单次给药，持续14天或28天。在体外研究中，我们评估了两种不同浓度的抗精神病药物对脾脏巨噬细胞3天培养物的影响。通过贴壁法分离大鼠脾脏巨噬细胞，并通过测量靶细胞P-815释放的51Cr来测定其细胞毒性活性。在体外研究中，所有抗精神病药物的两种浓度均未改变巨噬细胞的细胞毒性活性。在体内研究中，抗精神病药物（氯丙嗪2mg/kg、氟哌啶醇0.5mg/kg、舒必利50mg/kg）在单次注射后和14天后均增强了巨噬细胞的细胞毒性。研究结果表明，抗精神病药物的免疫调节作用主要取决于剂量和实验条件。
