Ishikawa S, Tozuka M, Hirota M, Sasaki Y, Okumura N, Furihata K, Katsuyama T
Central Clinical Laboratories, Shinshu University Hospital, Matsumoto.
Rinsho Byori. 1998 Jun;46(6):605-10.
Platelet aggregation, induced by agonist-mediated activation of membrane glycoprotein (GP) IIb/IIIa, and binding of fibrinogen to GPIIb/IIIa, is commonly analyzed using an aggregometer in the clinical laboratories. However, this method has a limitation to get precise results on the samples with small number of platelet (less than 100,000/1) or hyperlipidemia. Recently, flow cytometry has been used to evaluate platelet function due to the detection of fibrinogen binding to activated platelets using fluorescence labeled fibrinogen or anti-fibrinogen antibody. However, the appropriate rule for evaluation of the results has not been established yet. We converted a ratio of fibrinogen binding platelets to a velocity per unit concentration of ADP as follows: a difference of two ratios of fibrinogen binding platelets on neighboring two ADP concentrations was divided by a difference of ADP concentrations. It was considered to be a mean velocity between the two ADP concentrations. We adopted the range of ADP concentration, which gave the maximum velocity, as an index of platelet activation. If the peak of maximum velocity move toward lower or higher ADP concentration, it means hyper- or hypoactivation of the platelets, respectively. The objectivity of this method may make it a useful technique for clinical examination of platelet function.
由激动剂介导的膜糖蛋白（GP）IIb/IIIa激活所诱导的血小板聚集以及纤维蛋白原与GPIIb/IIIa的结合，在临床实验室中通常使用血小板聚集仪进行分析。然而，该方法在对血小板数量少（少于100,000/μl）或患有高脂血症的样本获取精确结果方面存在局限性。最近，由于使用荧光标记的纤维蛋白原或抗纤维蛋白原抗体检测纤维蛋白原与活化血小板的结合，流式细胞术已被用于评估血小板功能。然而，尚未建立评估结果的适当规则。我们将纤维蛋白原结合血小板的比率转换为每单位浓度ADP的速度，具体如下：相邻两个ADP浓度下纤维蛋白原结合血小板的两个比率之差除以ADP浓度之差。它被认为是两个ADP浓度之间的平均速度。我们采用给出最大速度的ADP浓度范围作为血小板活化的指标。如果最大速度峰值向较低或较高的ADP浓度移动，则分别意味着血小板的高活化或低活化。该方法的客观性可能使其成为临床检测血小板功能的有用技术。
