Oskam L, Pratlong F, Zijlstra E E, Kroon C C, Dedet I P, Kager P A, Schönian G, Ghalib H W, el-Hassan A M, Meredith S E
Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
Trans R Soc Trop Med Hyg. 1998 Jan-Feb;92(1):120-2. doi: 10.1016/s0035-9203(98)90982-8.
Twelve Leishmania isolates from visceral leishmaniasis patients in eastern Sudan were characterized using isoenzyme analysis, Southern blotting and polymerase chain reaction (PCR) 'fingerprinting'. Isoenzyme analysis revealed the presence of 3 zymodemes: MON-18, MON-30 and MON-82, corresponding to Leishmania donovani sensu stricto, L. infantum and L. archibaldi (still of uncertain taxonomic status), respectively. Southern blotting and PCR 'fingerprinting' revealed identical patterns for all 3 zymodemes, which were indistinguishable from those of L. donovani s.s.
采用同工酶分析、Southern印迹法和聚合酶链反应（PCR）“指纹图谱”技术，对来自苏丹东部内脏利什曼病患者的12株利什曼原虫分离株进行了特征分析。同工酶分析显示存在3种酶型：MON-18、MON-30和MON-82，分别对应于狭义杜氏利什曼原虫、婴儿利什曼原虫和阿奇巴尔德利什曼原虫（分类地位仍不确定）。Southern印迹法和PCR“指纹图谱”显示，所有3种酶型的图谱相同，与狭义杜氏利什曼原虫的图谱无法区分。
