从苏丹东部一个地方病疫源地分离出的利什曼原虫寄生虫的生化和分子特征
Biochemical and molecular characterization of Leishmania parasites isolated from an endemic focus in eastern Sudan.
作者信息
Oskam L, Pratlong F, Zijlstra E E, Kroon C C, Dedet I P, Kager P A, Schönian G, Ghalib H W, el-Hassan A M, Meredith S E
机构信息
Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
出版信息
Trans R Soc Trop Med Hyg. 1998 Jan-Feb;92(1):120-2. doi: 10.1016/s0035-9203(98)90982-8.
Twelve Leishmania isolates from visceral leishmaniasis patients in eastern Sudan were characterized using isoenzyme analysis, Southern blotting and polymerase chain reaction (PCR) 'fingerprinting'. Isoenzyme analysis revealed the presence of 3 zymodemes: MON-18, MON-30 and MON-82, corresponding to Leishmania donovani sensu stricto, L. infantum and L. archibaldi (still of uncertain taxonomic status), respectively. Southern blotting and PCR 'fingerprinting' revealed identical patterns for all 3 zymodemes, which were indistinguishable from those of L. donovani s.s.
采用同工酶分析、Southern印迹法和聚合酶链反应（PCR）“指纹图谱”技术，对来自苏丹东部内脏利什曼病患者的12株利什曼原虫分离株进行了特征分析。同工酶分析显示存在3种酶型：MON-18、MON-30和MON-82，分别对应于狭义杜氏利什曼原虫、婴儿利什曼原虫和阿奇巴尔德利什曼原虫（分类地位仍不确定）。Southern印迹法和PCR“指纹图谱”显示，所有3种酶型的图谱相同，与狭义杜氏利什曼原虫的图谱无法区分。
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