Murata M, Nakagawa M, Takahashi S
Department of Ophthalmology, Yamagata University School of Medicine Yamagata, Japan.
Ophthalmologica. 1998;212(5):331-3. doi: 10.1159/000027318.
Endothelin is a potent vasoactive agent and three isoforms--endothelin 1 (ET-1), endothelin 2 (ET-2) and endothelin 3 (ET-3)-- have been found in ocular tissues. However its source has not been determined. Therefore we have investigated the ET mRNA in the rat retina using reverse transcriptase with polymerase chain reaction. For ET-1, three retina samples were positive. For ET-2 and ET-3, all samples were negative. ET-1 mRNA was abundantly expressed compared with ET-2 and ET-3 mRNA (p < 0.05). Our study provides direct evidence that ET-1 is abundantly synthesized in the rat retina. The ET-1 within the retina could contribute to the regulation of the retinal circulation through vessel contraction.
内皮素是一种强效血管活性物质，在眼组织中已发现三种异构体——内皮素1（ET-1）、内皮素2（ET-2）和内皮素3（ET-3）。然而，其来源尚未确定。因此，我们使用逆转录酶和聚合酶链反应研究了大鼠视网膜中的ET mRNA。对于ET-1，三个视网膜样本呈阳性。对于ET-2和ET-3，所有样本均为阴性。与ET-2和ET-3 mRNA相比，ET-1 mRNA大量表达（p < 0.05）。我们的研究提供了直接证据，表明ET-1在大鼠视网膜中大量合成。视网膜内的ET-1可能通过血管收缩参与视网膜循环的调节。
