鼠肉孢子虫（顶复门）包囊裂殖子编码一种硫醇（半胱氨酸）蛋白酶的基因组DNA片段的扩增

Amplification of genomic DNA fragments of Sarcocystis muris (Apicomplexa) cyst merozoites encoding a thiol (cysteine) proteinase.

作者信息

Hansner T, Freyer B, Mehlhorn H, Rüger W

机构信息

Ruhr Universität Bochum, Arbeitsgruppe Molekulare Genetik, Germany.

出版信息

Parasitol Res. 1998 Jul;84(7):578-82. doi: 10.1007/s004360050452.

DOI:10.1007/s004360050452

PMID:9694376

Abstract

Parasites of the phylum Apicomplexa (Sporozoa) cause diseases such as malaria, toxoplasmosis, or intestinal coccidiosis. Invasive stages possess typical apical organelles such as dense granules that harbor a broad range of polypeptides that are believed to take part in the parasite-host cell interaction. In previous studies a 26-kDa polypeptide of dense granules from Sarcocystis muris cyst merozoites (bradyzoites) was characterized as a thiol (cysteine) proteinase. In this paper a method is demonstrated to amplify DNA fragments from genomic DNA of S. muris cyst merozoites by polymerase chain reaction, which probably code for the 26-kDa antigen.

摘要

顶复门（孢子虫纲）的寄生虫会引发诸如疟疾、弓形虫病或肠道球虫病等疾病。侵入阶段的寄生虫具有典型的顶端细胞器，如致密颗粒，其中含有多种多肽，据信这些多肽参与寄生虫与宿主细胞的相互作用。在先前的研究中，来自鼠肉孢子虫包囊裂殖子（缓殖子）致密颗粒的一种26 kDa多肽被鉴定为硫醇（半胱氨酸）蛋白酶。本文展示了一种通过聚合酶链反应从鼠肉孢子虫包囊裂殖子基因组DNA中扩增DNA片段的方法，这些片段可能编码26 kDa抗原。