Mochizuki T
Department of Dermatology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
Nihon Ishinkin Gakkai Zasshi. 1998;39(3):123-7. doi: 10.3314/jjmm.39.123.
Ultra-thin serial sections of mitotic cells of Cryptococcus neoformans were observed by transmission electron microscope and computer-aided reconstruction of three-dimensional models were performed to understand the kinetics of the nucleus and mitochondria during mitosis. The separation of chromosomes occured in the protrusion of the nucleus extending in the bud (=daughter cell), followed by one set of chromosomes being moved back into the mother cell. These findings were similar to those reported in the heterobasidiomycetous yeasts. No giant mitochondrion, composed of a coalescence of all the mitochondria, was found. Volumetric analysis revealed the fluctuations in the mitochondrial volume: total cytoplasmic volume ratio were minimum during mitosis. In addition, methods, usefulness and limitations of observation of ultra-thin serial sections for understanding the morphology of cells and intracellular organelles were reviewed.
通过透射电子显微镜观察新型隐球菌有丝分裂细胞的超薄连续切片，并进行计算机辅助的三维模型重建，以了解有丝分裂过程中细胞核和线粒体的动力学。染色体的分离发生在细胞核延伸到芽（=子细胞）中的突起处，随后一组染色体被移回母细胞。这些发现与在异担子菌酵母中报道的结果相似。未发现由所有线粒体合并而成的巨型线粒体。体积分析揭示了线粒体体积的波动：在有丝分裂期间，线粒体总体积与细胞质总体积之比最小。此外，还综述了用于理解细胞和细胞内细胞器形态的超薄连续切片观察方法、实用性和局限性。
