Katsuki S, Oui M, Takayama T, Takahashi Y, Shuichi N, Niitsu Y
Forth Department of Internal Medicine, School of Medicine, Sapporo Medical University, Japan.
Nihon Geka Gakkai Zasshi. 1998 Jun;99(6):379-84.
Aberrant crypt foci (ACF) are small lesions identifiable in whole-mount preparations of normal-appearing human colonic mucosa with the aid of methyleneblue staining under stereoscopic microscope. Highly frequent mutations of K-ras genes were found, and increased proliferative activities were also observed. Therefore, K-ras gene mutation may have some role in formation of ACF. Using rat experimental model, formation of ACF was shown to be enhanced by cancer promoters (such as secondary bile acids), and to be suppressed by chemopreventive agents (deoxycholic acid and aspirin). Based on these findings, ACF are considered to be precursors of colon cancer. We demonstrated that ACF are the precursors of adenoma-carcinoma sequence. Moreover, we showed that ACF may be suitable biomarkers of chemopreventive agents for colon cancers.
异常隐窝灶（ACF）是在立体显微镜下借助亚甲蓝染色，在外观正常的人类结肠黏膜全层标本中可识别的小病变。发现K-ras基因频繁突变，同时也观察到增殖活性增加。因此，K-ras基因突变可能在ACF的形成中起一定作用。使用大鼠实验模型，结果表明癌症启动剂（如次级胆汁酸）可增强ACF的形成，而化学预防剂（脱氧胆酸和阿司匹林）则可抑制其形成。基于这些发现，ACF被认为是结肠癌的前体。我们证明ACF是腺瘤-癌序列的前体。此外，我们还表明ACF可能是结肠癌化学预防剂的合适生物标志物。
