Simon P, Delsaut P, Lafontaine M, Morele Y, Nicot T
Institut National de Recherche et de Sécurité, Vandoeuvre, France.
J Chromatogr B Biomed Sci Appl. 1998 Aug 7;712(1-2):95-104. doi: 10.1016/s0378-4347(98)00139-x.
An extractionless method for determining aflatoxin M1 (AFM1), a major metabolite of aflatoxin B1 (AFB1), in human urine was developed. The biological fluid is injected directly into the chromatographic system after simple dilution and centrifugation. A pre-column, packed with a cation-exchange phase and coupled on-line to a column-switching liquid chromatography (LC) system, is used for sample pre-treatment and concentration. The analytes are non-selectively desorbed with the LC eluent and cleaned by means of a column-switching procedure. Pre-treatment and analysis were performed within 40 min. Average AFMI recovery reached 97% in the 10-100 ng/l range of urine. The detection limit of AFM1 in urine and milk was 2.5 ng/l for 1 ml of injected sample. A comparison with an immunoaffinity column clean-up and LC method was performed. The method was applied to determine AFM1 in the urine of AFB1 gavaged rats, and in the urine of both potentially exposed and supposedly unexposed workers. The method was also extended to milk.
开发了一种无需萃取的方法来测定人尿中黄曲霉毒素M1（AFM1），它是黄曲霉毒素B1（AFB1）的主要代谢产物。生物样品经简单稀释和离心后直接注入色谱系统。一个填充阳离子交换相并与柱切换液相色谱（LC）系统在线连接的预柱用于样品预处理和富集。分析物用LC洗脱液非选择性解吸，并通过柱切换程序进行净化。预处理和分析在40分钟内完成。在尿中10 - 100 ng/l范围内，AFM1的平均回收率达到97%。对于1 ml进样量的尿样和乳样，AFM1的检测限为2.5 ng/l。与免疫亲和柱净化和LC方法进行了比较。该方法应用于测定经AFB1灌胃大鼠的尿样以及可能接触和假定未接触的工人的尿样中的AFM1。该方法还扩展应用于牛奶。
