Bonnet M, Faulconnier Y, Fléchet J, Hocquette J F, Leroux C, Langin D, Martin P, Chilliard Y
Laboratoire sous-nutrition des ruminants, Inra, Theix, Saint-Genès-Champanelle, France.
Reprod Nutr Dev. 1998 May-Jun;38(3):297-307. doi: 10.1051/rnd:19980310.
The mechanisms involved in the nutritional regulation of genes encoding lipogenic (lipoprotein lipase (LPL) and fatty acid synthase (FAS)) and lipolytic (hormone-sensitive lipase (HSL)) enzymes were investigated by comparing the levels of the corresponding mRNAs in the adipose tissue (AT) of underfed or underfed-refed ewes and cows. Refeeding sharply increased LPL and FAS activities (19-25- and 6-8-fold, respectively) and moderately increased (2-4 fold) the activities of glucose-6-phosphate dehydrogenase (G6PDH), malic enzyme (ME) and glycerol-3-phosphate dehydrogenase (G3PDH). Northern blot analysis revealed three LPL transcripts and a single FAS transcript in cow and ewe AT. A single HSL mRNA was detected in cow AT and two transcripts in ewe AT. Refeeding sharply increased LPL and FAS mRNA levels, while restriction slightly increased (cows) or had no effect (ewes) on the HSL mRNA levels. This suggests that nutritional factors regulate sharply the expression of LPL and FAS genes by pretranslational mechanisms, but less clearly that of HSL gene.
通过比较饥饿或饥饿-再投喂母羊和母牛脂肪组织（AT）中相应mRNA的水平，研究了参与脂肪生成（脂蛋白脂肪酶（LPL）和脂肪酸合酶（FAS））和脂肪分解（激素敏感性脂肪酶（HSL））相关基因营养调控的机制。再投喂使LPL和FAS活性急剧增加（分别增加19 - 25倍和6 - 8倍），并使葡萄糖-6-磷酸脱氢酶（G6PDH）、苹果酸酶（ME）和甘油-3-磷酸脱氢酶（G3PDH）的活性适度增加（2 - 4倍）。Northern印迹分析显示，在母牛和母羊的AT中存在三种LPL转录本和一种单一的FAS转录本。在母牛AT中检测到一种单一的HSL mRNA，在母羊AT中检测到两种转录本。再投喂使LPL和FAS mRNA水平急剧增加，而限食使HSL mRNA水平略有增加（母牛）或没有影响（母羊）。这表明营养因子通过翻译前机制急剧调节LPL和FAS基因的表达，但对HSL基因表达的调节不太明显。
