Saito T, Sawamoto K, Okano H, Anderson D J, Mikoshiba K
Tsukuba Life Science Center, RIKEN, Ibaraki, Japan.
Dev Biol. 1998 Jul 15;199(2):216-25. doi: 10.1006/dbio.1998.8889.
Vertebrate neurogenesis involves sequential actions of transcription factors. neurogenins, encoding Atonal-related bHLH transcription factors, function as neuronal determination genes in Xenopus. neurogenins and antother bHLH factor gene, Mash1, are expressed in distinct subsets or areas of cells giving rise to neurons, suggesting that these genes play important roles to generate distinct populations of neurons. A mammalian homologue of BarH (MBH1) is expressed in a complementary pattern to Mash1 expression in the developing nervous system like neurogenins. Forced expression of MBH1 down-regulates expression of Mash1 and up-regulates neurogenin2/Math4A, a member of neurogenins, in P19 cells during neuronal differentiation. This suggests that MBH1 is a potential regulator of mammalian neural bHLH genes, thereby establishing distinct pathways of neuronal differentiation.
脊椎动物神经发生涉及转录因子的一系列作用。神经生成素编码与无调蛋白相关的bHLH转录因子，在非洲爪蟾中作为神经元决定基因发挥作用。神经生成素和另一个bHLH因子基因Mash1，在产生神经元的不同细胞亚群或区域中表达，这表明这些基因在生成不同神经元群体中发挥重要作用。BarH的哺乳动物同源物（MBH1）在发育中的神经系统中以与Mash1表达互补的模式表达，就像神经生成素一样。在神经元分化过程中，MBH1的强制表达下调了P19细胞中Mash1的表达，并上调了神经生成素2/Math4A（神经生成素的一个成员）的表达。这表明MBH1是哺乳动物神经bHLH基因的潜在调节因子，从而建立了不同的神经元分化途径。
