在意大利弱β-溶血人肠道螺旋体分离株中检测染色体外DNA。

Detection of extrachromosomal DNA in Italian isolates of weakly beta-haemolytic human intestinal spirochaetes.

作者信息

Cattani P, Dettori G, Calderaro A, Grillo R, Fadda G, Chezzi C

机构信息

Institute of Microbiology, Faculty of Medicine and Surgery, Catholic University of the Sacred Heart, Rome, Italy.

出版信息

New Microbiol. 1998 Jul;21(3):241-8.

PMID:9699203

Abstract

Weakly beta-haemolytic human intestinal spirochaetes (w beta HIS) isolated in Italy showed an extrachromosomal band migrating at 4.3 kb after agarose gel electrophoresis of total DNA. The band was observed within 4 strains (HRM2, HRM4, HRM7 and HRM14) among the 7 w beta HIS analysed and was resistant to proteinase K and RNase treatment, whereas after purification it was completely digested by incubation with DNaseI. The origin, the structure and the significance of this extrachromosomal DNA are at present unknown. In addition to w beta HIS, the reference strain P18A of Serpulina hyodysenteriae was comparatively analysed and a 6.5 Kb extrachromosomal DNA element was found, as expected. This finding proves that the experimental conditions we used while searching for extrachromosomal DNA within w beta HIS were appropriate. To the best of our knowledge, this is the first report on the detection of extrachromosomal DNA from w beta HIS.

摘要

在意大利分离出的弱β-溶血人肠道螺旋体（wβHIS），经总DNA琼脂糖凝胶电泳后，显示出一条迁移至4.3 kb的染色体外条带。在所分析的7株wβHIS中，在4株（HRM2、HRM4、HRM7和HRM14）中观察到了该条带，它对蛋白酶K和核糖核酸酶处理具有抗性，而纯化后，经与脱氧核糖核酸酶I孵育可将其完全消化。目前，这种染色体外DNA的来源、结构和意义尚不清楚。除了wβHIS外，还对猪痢疾蛇形螺旋体的参考菌株P18A进行了比较分析，正如预期的那样，发现了一个6.5 Kb的染色体外DNA元件。这一发现证明，我们在wβHIS中寻找染色体外DNA时所使用的实验条件是合适的。据我们所知，这是关于从wβHIS中检测到染色体外DNA的首次报告。