两种猪骨骼肌肌球蛋白重链亚型的分离与定位

Isolation and mapping of two porcine skeletal muscle myosin heavy chain isoforms.

作者信息

Davoli R, Zambonelli P, Bigi D, Fontanesi L, Russo V

机构信息

DIPROVAL, University of Bologna, Reggio Emilia, Italy.

出版信息

Anim Genet. 1998 Apr;29(2):91-7. doi: 10.1046/j.1365-2052.1998.00275.x.

DOI:10.1046/j.1365-2052.1998.00275.x

PMID:9699268

Abstract

The present paper describes the isolation and linkage mapping of two isoforms of skeletal muscle myosin heavy chain in pig. Two partial cDNAs (pAZMY4 and pAZMY7), coding for the porcine myosin heavy chain-2B and -beta respectively, have been isolated from a pig skeletal muscle cDNA library. Four RFLPs were detected with the putative porcine skeletal myosin heavy chain-2B probe (pAZMY4) and one RFLP was identified with the putative myosin heavy chain-beta probe (pAZMY7). Two myosin heavy chain loci were mapped by linkage analysis performed with the five RFLPs against the PiGMaP linkage consortium ResPig database: the MYH1 locus, which identifies the fast skeletal muscle myosin heavy chain gene cluster, was located at the end of the map of porcine chromosome 12, while the MYH7 locus, which identifies the myosin heavy chain-alpha/-beta gene cluster, was assigned to the long arm of porcine chromosome 7.

摘要

本文描述了猪骨骼肌肌球蛋白重链两种同工型的分离及连锁图谱构建。分别从猪骨骼肌cDNA文库中分离出两个部分cDNA（pAZMY4和pAZMY7），它们分别编码猪肌球蛋白重链-2B和-β。用推定的猪骨骼肌肌球蛋白重链-2B探针（pAZMY4）检测到4个限制性片段长度多态性（RFLP），用推定的肌球蛋白重链-β探针（pAZMY7）鉴定出1个RFLP。利用这5个RFLP针对PiGMaP连锁联盟的ResPig数据库进行连锁分析，对两个肌球蛋白重链基因座进行了定位：识别快速骨骼肌肌球蛋白重链基因簇的MYH1基因座位于猪12号染色体图谱的末端，而识别肌球蛋白重链-α/-β基因簇的MYH7基因座被定位到猪7号染色体的长臂上。