大鼠孕期子宫平滑肌中的肌球蛋白同工型

Myosin isoforms in uterine smooth muscle during pregnancy in rat.

作者信息

Kumar R, Malhotra R K, Katoch S S

机构信息

Department of Biosciences, Himachal Pradesh University, Shimla, India.

出版信息

Indian J Biochem Biophys. 1998 Feb;35(1):28-33.

PMID:9699416

Abstract

Effects of uterine stretching and physiological hypertrophy on myosin isozyme were investigated in rat during pregnancy. Both nonpregnant and pregnant rat uteri express a single myosin band on native gels. Analysis of native myosin under denaturing conditions revealed two myosin heavy chains (MHCs) with molecular mass of 204 and 200 kDa respectively. Filamin, a 240 kDa protein co-electrophoreses with myosin on native gels. No correlation is found between regulatory myosin light chain phosphorylation and pattern of myosin isozymes or the MHC. The results suggest that uterine stretching and physiological hypertrophy during pregnancy do not induce any changes in uterine myosin isozyme.

摘要

在大鼠孕期研究了子宫伸展和生理性肥大对肌球蛋白同工酶的影响。未怀孕和怀孕大鼠的子宫在天然凝胶上均表达单一的肌球蛋白条带。在变性条件下对天然肌球蛋白进行分析，发现两条肌球蛋白重链（MHC），分子量分别为204 kDa和200 kDa。细丝蛋白是一种240 kDa的蛋白质，在天然凝胶上与肌球蛋白共电泳。未发现调节性肌球蛋白轻链磷酸化与肌球蛋白同工酶模式或MHC之间存在相关性。结果表明，孕期子宫伸展和生理性肥大不会引起子宫肌球蛋白同工酶的任何变化。