Kurien B T, Scofield R H
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, USA.
Indian J Biochem Biophys. 1998 Feb;35(1):41-7.
Multiple nuclear dot (MND), or pseudocentromere, anti-nuclear antibody (ANA) is an uncommon pattern associated primarily with primary biliary cirrhosis (PBC) and anti-mitochondrial antibody (AMA). A 53 kDa antigen with an apparent molecular mass of 100 kDa as found on sodium dodecyl sulphate-polyacrylamide gel electrophoresis is thought to be responsible for the uncommon pattern. This study analyzes sera from 21 patients without PBC or AMA that produced the uncommon MND ANA immunofluorescence pattern. Diseases present include lupus, rheumatoid arthritis and scleroderma. On immunoblotting nineteen of 21 (91%) bound a 70 kDa protein. Western blot analysis showed that this nuclear antigen was different from pyruvate dehydrogenase, p80 coilin and the antigen responsible for MND ANA in those with PBC. Affinity purified anti-70 kDa reproduced the MND ANA immunofluorescence pattern. Thus, the MND ANA in patients without PBC/AMA is associated with binding to a 70 kDa nuclear protein and not with a 53 kDa antigen (that runs at 100 kDa) found in those with MND and PBC/AMA. The data demonstrate the MND antigen without PBC/AMA is immunologically distinct from the pattern when found with PBC/AMA.
多核点（MND）或假着丝粒抗核抗体（ANA）是一种不常见的模式，主要与原发性胆汁性肝硬化（PBC）和抗线粒体抗体（AMA）相关。在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳上发现的一种表观分子量为100 kDa但实际分子量为53 kDa的抗原被认为是导致这种不常见模式的原因。本研究分析了21例无PBC或AMA但产生不常见的MND ANA免疫荧光模式的患者的血清。这些患者所患疾病包括狼疮、类风湿性关节炎和硬皮病。免疫印迹显示，21例中有19例（91%）与一种70 kDa的蛋白质结合。蛋白质印迹分析表明，这种核抗原不同于丙酮酸脱氢酶、p80卷曲螺旋蛋白以及PBC患者中导致MND ANA的抗原。亲和纯化的抗70 kDa抗体重现了MND ANA免疫荧光模式。因此，无PBC/AMA患者的MND ANA与一种70 kDa核蛋白的结合有关，而与MND和PBC/AMA患者中发现的53 kDa抗原（在凝胶上迁移为100 kDa）无关。数据表明，无PBC/AMA时的MND抗原在免疫上与伴有PBC/AMA时的模式不同。
