Mancinelli L, Lugaro G, De Angelis L, Gianfranceschi G L
Dipartimento di Biologia Cellulare e Molecolare sezione di Fisiologia e Biofisica, Università di Perugia, Italy.
Mol Biol Rep. 1998 Jul;25(3):163-72. doi: 10.1023/a:1006813814125.
Structural features of a class of chromatin peptides are studied in the aim of understanding their mechanism of action. They have been reported as a family of small acidic peptides that can affect cell proliferation and RNA transcription. Mass spectrometry analysis has suggested some molecular models of possible sequences that might be present in this group of peptides. These sequences have been synthesised and their chromatographic and electrophoretic behaviour is compared with that obtained from peptides extracted from pea bud chromatin. In this way electric charge and hydrophilic properties of the native peptides are evaluated. On the basis of these data and those obtained from further mass spectrum analysis new models for native peptides are proposed.
为了理解一类染色质肽的作用机制，对其结构特征进行了研究。它们被报道为一类能影响细胞增殖和RNA转录的小酸性肽家族。质谱分析提出了该类肽可能存在的一些分子序列模型。已合成这些序列，并将其色谱和电泳行为与从豌豆芽染色质中提取的肽的行为进行比较。通过这种方式评估天然肽的电荷和亲水性质。基于这些数据以及进一步质谱分析获得的数据，提出了天然肽的新模型。
