Rodríguez E, Antillón F
Facultad de Microbiología, Universidad de Costa Rica.
Rev Biol Trop. 1989 Jun;37(1):69-73.
Bivalves, mud, and surface water were collected at three different sites of the Gulf of Nicoya, Costa Rica, in search of Aeromonas spp. and Plesiomonas shigelloides. For their isolation, these bacteria were enriched in alkaline peptone water and streaked on MacConkey agar and on brilliant green bile inositol agar. This was followed by the biochemical tests necessary for their identification. Thirty-five strains of A. hydrophila, 58 of A. caviae, 43 of A. sobria, and 7 of P. shigelloides were isolated. None of these predominated nor was there any indication of a seasonal distribution along the 15 month's duration of the study. Seven strains of A. hydrophila and two of A. sobria showed the biochemical characteristics associated with toxin production (positive Voges-Proskauer and lysine decarboxylase tests). These species are widely distributed in the gulf and there is risk of contracting an infection while bathing or when eating raw bivalves from this area.
在哥斯达黎加尼科亚湾的三个不同地点采集了双壳贝类、淤泥和地表水,以寻找气单胞菌属和类志贺邻单胞菌。为了分离这些细菌,将它们在碱性蛋白胨水中进行富集培养,然后划线接种于麦康凯琼脂和煌绿胆盐肌醇琼脂上。接下来进行鉴定所需的生化试验。分离出35株嗜水气单胞菌、58株豚鼠气单胞菌、43株温和气单胞菌和7株类志贺邻单胞菌。在为期15个月的研究期间,这些菌株均未占主导地位,也没有季节性分布的迹象。7株嗜水气单胞菌和2株温和气单胞菌表现出与毒素产生相关的生化特征(Voges-Proskauer试验和赖氨酸脱羧酶试验呈阳性)。这些菌种在海湾中广泛分布,在该地区游泳或食用生的双壳贝类时有感染的风险。