Welfle H, Henkel B, Bielka H
Acta Biol Med Ger. 1976;35(3-4):401-11.
Proteins of rat liver ribosomal subunits were successively split off by stepwise treatment of the particles with various concentrations of LiC1 (0.4--1.0 M) or KC1 (0.6--1.5 M). Split proteins and core particle proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. The LiC1 concentrations were estimated at which the individual proteins are split off. The proteins of 40S and 60S subunits were classified in 4 and 5 groups, respectively, according to their splitting behavior. The effect of LiC1 and KC1 is similar, but higher concentrations of KC1 than of LiC1 are required to obtain comparable split protein patterns. Evidence for structural changes of the core particles during the split procedures was obtained.
通过用不同浓度的LiC1（0.4 - 1.0M）或KC1（0.6 - 1.5M）对大鼠肝脏核糖体亚基颗粒进行逐步处理，相继分离出核糖体亚基的蛋白质。通过二维聚丙烯酰胺凝胶电泳分析分离出的蛋白质和核心颗粒蛋白质。估算了分离出各个蛋白质时的LiC1浓度。根据其分离行为，40S和60S亚基的蛋白质分别分为4组和5组。LiC1和KC1的作用相似，但需要比LiC1更高浓度的KC1才能获得可比的分离蛋白质模式。获得了在分离过程中核心颗粒结构变化的证据。
