An Y H, Friedman R J
Department of Orthopedic Surgery, Medical University of South Carolina, Charleston 29425, USA.
J Invest Surg. 1998 Mar-Apr;11(2):139-46. doi: 10.3109/08941939809032193.
Prosthetic infection following total joint replacement can have catastrophic results both physically and psychologically for patients, leading to complete failure of the arthroplasty, possible amputation, prolonged hospitalization, and even death. Although with the use of prophylactic antibiotics and greatly improved operating room techniques the infection rate has decreased markedly during the years, challenges still remain for better preventive and therapeutic measures. In this review the in vivo experimental methods for studies of prosthetic infection are discussed, concentrating on (1) the animal models that have been established and the use of these animal models for studies of pathogenesis of bacteria, behavior of biofilm, effect of biomaterials on prosthetic infection rate, and the effect of infection on biomaterial surfaces, and (2) how to design and conduct an animal model of orthopedic prosthetic infection including animal selection, implant fabrication, bacterial inoculation, surgical technique, and the methods for evaluating the results.
全关节置换术后的假体感染对患者的身体和心理都会造成灾难性后果，可导致关节成形术完全失败、可能需要截肢、住院时间延长甚至死亡。尽管近年来随着预防性抗生素的使用以及手术室技术的大幅改进，感染率已显著下降，但在寻求更好的预防和治疗措施方面仍面临挑战。在这篇综述中，将讨论用于假体感染研究的体内实验方法，重点关注：（1）已建立的动物模型以及这些动物模型在细菌发病机制研究、生物膜行为研究、生物材料对假体感染率的影响研究以及感染对生物材料表面的影响研究中的应用；（2）如何设计和开展骨科假体感染动物模型，包括动物选择、植入物制作、细菌接种、手术技术以及结果评估方法。
