Kimura Y, Hayashida K, Ishibashi H, Niho Y, Akazawa K, Yanagi Y
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Med Virol. 1998 Sep;56(1):25-32.
In order to identify reliable predictive factors for the response to interferon (IFN) therapy, we examined 52 patients with chronic hepatitis C virus (HCV) infection who underwent IFN therapy. Titers of free virions that were not complexed with antibodies were determined by immunoprecipitation of the pretreatment sera with antihuman immunoglobulin. Cell attachment titers were determined by inoculating HPB-Ma cells, a human T-cell line, with the patients' pretreatment sera. A sustained remission was achieved in 9 out of 28 patients with genotype 1b infection and in 18 out of 24 patients with genotype 2 infection. The free virion titers and the cell attachment titers correlated well with the serum HCV RNA levels in patients with genotype 1b infection (r = 0.817, P < 0.001; r = 0.802, P < 0.001, respectively), but not in those with genotype 2 infection. They exhibited a high correlation with the responsiveness to IFN in all patients combined (P < 0.001). A multiple logistic regression analysis was performed to identify the factor most significantly associated with the response to IFN among all confounding factors. The analysis indicated that the cell attachment is the most reliable predictive factor regarding a favorable response to IFN therapy in patients with chronic HCV infection.
为了确定干扰素（IFN）治疗反应的可靠预测因素，我们检查了52例接受IFN治疗的慢性丙型肝炎病毒（HCV）感染患者。通过用抗人免疫球蛋白对治疗前血清进行免疫沉淀来测定未与抗体结合的游离病毒粒子的滴度。通过用患者的治疗前血清接种人T细胞系HPB-Ma细胞来测定细胞附着滴度。28例基因型1b感染患者中有9例实现了持续缓解，24例基因型2感染患者中有18例实现了持续缓解。基因型1b感染患者的游离病毒粒子滴度和细胞附着滴度与血清HCV RNA水平相关性良好（r分别为0.817，P < 0.001；r为0.802，P < 0.001），但基因型2感染患者并非如此。在所有患者中，它们与对IFN的反应性高度相关（P < 0.001）。进行了多因素logistic回归分析，以确定在所有混杂因素中与IFN反应最显著相关的因素。分析表明，细胞附着是慢性HCV感染患者对IFN治疗产生良好反应的最可靠预测因素。
