Itoh Y, Tomita M, Tanahashi N, Takeda H, Yokoyama M, Fukuuchi Y
Department of Neurology, Keio University School of Medicine, Tokyo-to, Japan.
Thromb Res. 1998 Jul 1;91(1):15-21. doi: 10.1016/s0049-3848(98)00054-1.
Secondary thrombus formation following arterial occlusion is suggested to play an important role in the exacerbation of ischemic organ damage. We investigated the effect of thrombin on endothelial cells from the aspect of morphological changes and induction of platelet adhesion to the endothelial cells. Using a video-enhanced contrast microscopy, we observed human aortic endothelial cells (HAEC) following perfusion of human alpha-thrombin of 1.0 U/ml (n = 7) or vehicle (n = 7) for 30 minutes. The endothelial cells began to shrink 15 minutes after thrombin administration. Gaps between the cells were formed. The cells became rearranged orderly in the same direction 30 minutes later. In another study, following pretreatment with human alpha-thrombin 1.0 U/ml (n = 10) or vehicle (n = 7) for 20 minutes and washout, platelets were perfused over HAEC for 30 minutes. Platelets adhered directly to thrombin-treated endothelial cells and became flat on the endothelial cells. Then other platelets were observed to approach to the flattened platelets and aggregated onto it. After washout of floating platelets, adhesion of platelets was further confirmed. These results suggest that thrombin may be involved in the endothelial damage and formation of platelet thrombi on the endothelial cells after blood flow disturbance.
动脉闭塞后继发性血栓形成被认为在缺血性器官损伤的加重中起重要作用。我们从形态学变化以及诱导血小板黏附于内皮细胞方面研究了凝血酶对内皮细胞的影响。使用视频增强对比显微镜，我们观察了在灌注1.0 U/ml人α凝血酶（n = 7）或赋形剂（n = 7）30分钟后的人主动脉内皮细胞（HAEC）。给予凝血酶后15分钟内皮细胞开始收缩。细胞间形成间隙。30分钟后细胞沿同一方向有序重排。在另一项研究中，用1.0 U/ml人α凝血酶（n = 10）或赋形剂（n = 7）预处理20分钟并冲洗后，将血小板灌注到HAEC上30分钟。血小板直接黏附于经凝血酶处理的内皮细胞并在内皮细胞上变扁平。然后观察到其他血小板靠近扁平的血小板并聚集在其上。冲洗掉漂浮的血小板后，进一步证实了血小板的黏附。这些结果表明，凝血酶可能参与血流紊乱后内皮损伤以及内皮细胞上血小板血栓的形成。
