Manz H J, Robertson D M, Haas R A, Meyers N
Acta Neuropathol. 1976 Sep 15;36(1):47-56. doi: 10.1007/BF00685147.
Immature female rats were subjected to acute dietary deficiency of thiamine. An autoradiographic method was used in the semi-quantitative determination of concentration of 1-aminocyclopentane-1-carboxylic acid-carboxylic-14C (cycloleucine) in brainstem regions after intravenous administration of tracer quantities. The time course of tissue concentrations was followed and compared with that of normal and isocaloric control animals. Our data indicate that thiamine deficiency of sufficient magnitude to induce brainstem lesions has an effect on the transport of cycloleucine. The initial 2 min values in the nuclear areas are appreciably reduced whereas the 6 min values are significantly elevated as compared with controls; no change was demonstrated in the white matter of the inferior cerebellar peduncle. The effect on transport appears to be more on the mechanism of efflux than of influx in terms of blood brain barrier function.
将未成熟雌性大鼠置于硫胺素急性饮食缺乏状态。采用放射自显影法，在静脉注射示踪量的1-氨基环戊烷-1-羧酸-羧基-¹⁴C（环亮氨酸）后，对脑干区域的其浓度进行半定量测定。追踪组织浓度的时间进程，并与正常和等热量对照动物的进行比较。我们的数据表明，足以诱发脑干病变的硫胺素缺乏会对环亮氨酸的转运产生影响。与对照组相比，核区域最初2分钟的值明显降低，而6分钟的值显著升高；小脑下脚白质未显示出变化。就血脑屏障功能而言，对转运的影响似乎更多体现在流出机制而非流入机制上。
