Even-Ram S, Uziely B, Cohen P, Grisaru-Granovsky S, Maoz M, Ginzburg Y, Reich R, Vlodavsky I, Bar-Shavit R
Department of Oncology, Hadassah-Hebrew University Hospital, Jerusalem, Israel.
Nat Med. 1998 Aug;4(8):909-14. doi: 10.1038/nm0898-909.
Although the involvement of soluble and matrix-immobilized proteases in tumor cell invasion and metastasis is well recognized, the role of proteolytically activated cell surface receptors has not been elucidated. We report here that thrombin receptor, a member of the protease-activated receptor family, is preferentially expressed in highly metastatic human breast carcinoma cell lines and breast carcinoma biopsy specimens. Introduction of thrombin receptor antisense cDNA considerably inhibited the invasion of metastatic breast carcinoma cells in culture through a reconstituted basement membrane. During placental implantation of the human embryo, thrombin receptor is transiently expressed in the invading cytotrophoblasts. These results emphasize the involvement of thrombin receptor in cell invasion associated with tumor progression and normal embryonic development.
虽然可溶性蛋白酶和固定于基质的蛋白酶参与肿瘤细胞侵袭和转移已得到广泛认可，但蛋白水解激活的细胞表面受体的作用尚未阐明。我们在此报告，凝血酶受体作为蛋白酶激活受体家族的一员，在高转移性人乳腺癌细胞系和乳腺癌活检标本中优先表达。导入凝血酶受体反义cDNA可显著抑制培养的转移性乳腺癌细胞通过重组基底膜的侵袭。在人类胚胎的胎盘植入过程中，凝血酶受体在侵入的细胞滋养层中短暂表达。这些结果强调了凝血酶受体参与与肿瘤进展和正常胚胎发育相关的细胞侵袭。
