海洋需氧固氮单细胞蓝细菌蓝藻菌属ATCC 51142中的限制-修饰系统。

Restriction-modification systems in the marine, aerobic, nitrogen-fixing unicellular cyanobacterium Cyanothece sp. ATCC 51142.

作者信息

Soper BW, Hollister WR, Reddy KJ

机构信息

Department of Biological Sciences, State University of New York at Binghamton, Binghamton, NY 13902-6000, USA

出版信息

J Mar Biotechnol. 1998 Aug;6(3):183-5.

PMID:9701642

Abstract

Cyanothece sp. ATCC 51142 possesses six host restriction-modification systems (HRMS), and these HRMS were designated as Csp68KI to Csp68KVI. So far, four restriction enzymes have been characterized biochemically, and the other two were deduced based on the cloning of corresponding DNA methyltransferase genes M. Csp68KIV and M. Csp68KV from the Cyanothece genome. Csp68KI, Csp68KII, Csp68KIII, Csp68KIV, Csp68KV, and Csp68KVI are isoschizomers of AvaII, AsuII, AvaIII, MspI, HaeIII, and FnuDII, respectively. The cleavage specificities for Csp68KI, Csp68KII, Csp68KIII, and Csp68KVI were characterized. The Cyanothece restriction enzymes showed different temperature and salt requirements for their optimal activity. For example, the restriction enzyme Csp68KII functions optimally at 50 degreesC and Csp68KIII requires higher salt for its activity.

摘要

蓝细菌属（Cyanothece）的ATCC 51142菌株拥有六个宿主限制修饰系统（HRMS），这些HRMS被命名为Csp68KI至Csp68KVI。到目前为止，已对四种限制酶进行了生化特性鉴定，另外两种则是基于从蓝细菌基因组中克隆出相应的DNA甲基转移酶基因M. Csp68KIV和M. Csp68KV推断出来的。Csp68KI、Csp68KII、Csp68KIII、Csp68KIV、Csp68KV和Csp68KVI分别是AvaII、AsuII、AvaIII、MspI、HaeIII和FnuDII的同裂酶。已对Csp68KI、Csp68KII、Csp68KIII和Csp68KVI的切割特异性进行了表征。蓝细菌限制酶在最适活性时表现出不同的温度和盐需求。例如，限制酶Csp68KII在50℃时活性最佳，而Csp68KIII的活性需要更高的盐浓度。