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[空白弹药筒中蜡样芽孢杆菌的形态学检测]

[Morphologic detection of Bacillus cereus in blank cartridges].

作者信息

Rothschild M A, Mülling C

机构信息

Institut für Rechtsmedizin, Freien Universität Berlin.

出版信息

Arch Kriminol. 1998 May-Jun;201(5-6):172-81.

PMID:9701752
Abstract

Wound infections after gunshot wounds from live ammunition can produce serious complications. It is well known that projectiles per se are neither sterile nor does their firing cause sterilization. The germs on the surface of a projectile enter the body together with the projectile and are thus introduced into the wound together with skin bacteria. However it is less known that wound infections can occur in wounds caused by the gas jet from blank ammunition (mainly from shots at very close range). In such ammunition without a projectile, the propellant particles are usually contaminated with bacteria which find their way into the wound together with skin germs. In previous investigations, we have microbiologically detected the species Bacillus cereus in the propellant of blank cartridges. In the present study, we have applied scanning electron microscopic methods to find out which areas of the blank cartridges are colonized by these bacteria. For this purpose 20 blank cartridges, each from 4 different manufacturers, were electronmicroscopically examined. B. cereus only found on the surface of intact nitrocellulose particles but not in the interior of broken prepared propellant particles. Bacterial structures were not morphologically identified on black powder particles.

摘要

实弹枪伤后的伤口感染会引发严重并发症。众所周知,子弹本身并非无菌,发射也不会使其灭菌。子弹表面的细菌会随子弹一同进入人体,进而与皮肤细菌一起被带入伤口。然而,鲜为人知的是,空包弹产生的燃气射流造成的伤口(主要是近距离射击)也会发生伤口感染。在这种没有子弹的弹药中,推进剂颗粒通常被细菌污染,这些细菌会与皮肤细菌一起进入伤口。在之前的研究中,我们通过微生物学方法在空包弹的推进剂中检测到了蜡样芽孢杆菌。在本研究中,我们应用扫描电子显微镜方法来查明这些细菌在空包弹的哪些部位定殖。为此,对来自4个不同制造商的20枚空包弹进行了电子显微镜检查。蜡样芽孢杆菌仅在完整的硝化纤维素颗粒表面发现,而在破碎的预制推进剂颗粒内部未发现。在黑火药颗粒上未通过形态学鉴定出细菌结构。

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