Slegtenhorst-Eegdeman K E, Verhoef-Post M, Parvinen M, Grootegoed J A, Themmen A P
Department of Endocrinology and Reproduction, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The Netherlands.
Mol Hum Reprod. 1998 Jul;4(7):649-56. doi: 10.1093/molehr/4.7.649.
In immature rat Sertoli cells, leucine-rich primary response gene 1 (LRPR1) represents a follicle stimulating hormone (FSH)-responsive gene; the function of the encoded protein is not yet known. LRPR1 mRNA expression is up-regulated very rapidly and specifically by FSH, both in cultured Sertoli cells and in vivo in regulation in more detail, in testis and ovary of fetal, immature, and adult rats. In addition, we have studied the expression of FSH receptor (FSHR) mRNA in relation to LRPR1 mRNA expression. In rat testis, LRPR1 mRNA and FSHR mRNA followed a similar expression pattern, during postnatal development and also at different stages of the spermatogenic cycle in the adult rat. Furthermore, after short-term challenge of the FSH signal transduction pathway in intact immature rats by injection with a relatively high dose of FSH, an inverse relationship between LRPR1 mRNA (up-regulation) and FSHR mRNA expression (down-regulation) was observed. Similar studies in the ovary provided completely different results. LRPR1 mRNA in the postnatal ovary is present well before expression of FSHR mRNA can be first detected. In addition, incubation of ovaries of immature rats with FSH or dibutyryl cyclic AMP (dbcAMP) did not result in up-regulation of LRPR1 mRNA expression. During fetal development, the LRPR1 mRNA expression pattern involved many more tissues, in contrast to the relatively tissue-specific expression of LRPR1 mRNA in gonads of 21 day old and adult rats. Moreover, LRPR1 mRNA expression could be detected as early as 12.5 days post-coitum, whereas FSHR mRNA is absent at this stage of fetal development. We concluded that the pronounced regulation of LRPR1 by FSH observed in the immature rat testis does not occur in the ovary. Furthermore, in the ovary LRPR1 mRNA expression does not appear to be dependent on FSH action. Finally, the LRPR1 gene product may play a general role during fetal development.
在未成熟大鼠的支持细胞中，富含亮氨酸的初级反应基因1（LRPR1）是一种促卵泡激素（FSH）反应基因；其编码蛋白的功能尚不清楚。在培养的支持细胞以及胎儿、未成熟和成年大鼠的睾丸及卵巢中，FSH均可非常迅速且特异性地上调LRPR1 mRNA的表达。此外，我们还研究了FSH受体（FSHR）mRNA与LRPR1 mRNA表达之间的关系。在大鼠睾丸中，LRPR1 mRNA和FSHR mRNA在出生后发育过程以及成年大鼠生精周期的不同阶段呈现相似的表达模式。此外，在完整未成熟大鼠中通过注射相对高剂量的FSH对FSH信号转导通路进行短期刺激后，观察到LRPR1 mRNA（上调）与FSHR mRNA表达（下调）之间呈负相关。在卵巢中进行的类似研究则得出了完全不同的结果。出生后卵巢中的LRPR1 mRNA在首次检测到FSHR mRNA表达之前就已大量存在。此外，用FSH或二丁酰环磷腺苷（dbcAMP）孵育未成熟大鼠的卵巢并不会导致LRPR1 mRNA表达上调。在胎儿发育过程中，LRPR1 mRNA的表达模式涉及更多组织，这与21日龄和成年大鼠性腺中LRPR1 mRNA相对组织特异性的表达形成对比。此外，早在交配后12.5天就能检测到LRPR1 mRNA的表达，而在此胎儿发育阶段FSHR mRNA并不存在。我们得出结论，在未成熟大鼠睾丸中观察到的FSH对LRPR1的显著调节在卵巢中并不发生。此外，在卵巢中LRPR1 mRNA的表达似乎不依赖于FSH的作用。最后，LRPR1基因产物可能在胎儿发育过程中发挥普遍作用。
