Thakur A, Willcox M D
Cornea and Contact Lens Research Unit, University of New South Wales, Sydney, Australia.
Exp Eye Res. 1998 Jul;67(1):9-19. doi: 10.1006/exer.1998.0480.
Contact lens induced acute red eye (CLARE) and contact lens induced peripheral ulcer (CLPU) are among the most common contact lens induced inflammatory reactions. Both CLARE and CLPU are characterized by corneal infiltration which indicates the presence of chemoattractants and other inflammatory mediators. The aim of this study was to characterize the cytokine and chemotactic lipid inflammatory mediator profile in the tears of people experiencing CLARE or CLPU. Cytokines IL-1 beta, IL-6, IL-8, GM-CSF and LTB4 in tears were measured by antibody sandwich and competition inhibition enzyme-linked immunosorbent assays (ELISA). Platelet activating factor-like activity was measured by a degranulation assay by measuring the release of labelled serotonin from platelets. The functional role GM-CSF and chemoattractants were determined by flow cytometry and chemotaxis. Increased levels of cytokines and chemoattractants were detected in both CLARE and CLPU tears. CLPU tears showed increased levels of LTB4 (P = 0.002) and PAF-like activity (P = 0.047) whereas CLARE tears showed increased levels of GM-CSF (P = 0.002). IL-8 (P < 0.05). LTB4 (P = 0.002) and PAF-like activity (P = 0.047) compared to control tears. Flow cytometric analysis revealed that incubation of PMN with CLARE tears increased the number of IgA receptors indicating that the GM-CSF in CLARE tears was active. Combinations of suboptimal concentrations (which were found in CLARE and CLPU tears) of IL-8 with either LTB4 or PAF significantly (P < 0.0001) enhanced the chemotactic activity for PMN compared to their individual effects. Our data highlight the possible pathophysiological roles of these inflammatory mediators in leukocyte recruitment and activation during ocular inflammatory responses. The results suggests that GM-CSF, IL-8 and LTB4 are active during corneal pathology and LTB4 or IL-8 may maintain the contact lens induced PMN response in vivo.
隐形眼镜诱发的急性红眼（CLARE）和隐形眼镜诱发的周边溃疡（CLPU）是最常见的隐形眼镜诱发的炎症反应。CLARE和CLPU均以角膜浸润为特征，这表明存在趋化因子和其他炎症介质。本研究的目的是表征CLARE或CLPU患者泪液中的细胞因子和趋化脂质炎症介质谱。通过抗体夹心和竞争抑制酶联免疫吸附测定（ELISA）测量泪液中的细胞因子IL-1β、IL-6、IL-8、GM-CSF和LTB4。通过测量血小板中标记的血清素的释放，通过脱颗粒测定来测量血小板活化因子样活性。通过流式细胞术和趋化性确定GM-CSF和趋化因子的功能作用。在CLARE和CLPU泪液中均检测到细胞因子和趋化因子水平升高。CLPU泪液显示LTB4水平升高（P = 0.002）和PAF样活性升高（P = 0.047），而CLARE泪液显示GM-CSF水平升高（P = 0.002）、IL-8（P < 0.05）、LTB4（P = 0.002）和PAF样活性升高（P = 0.047），与对照泪液相比。流式细胞术分析显示，PMN与CLARE泪液孵育增加了IgA受体的数量，表明CLARE泪液中的GM-CSF具有活性。与它们各自的作用相比，IL-8与LTB4或PAF的次优浓度组合（在CLARE和CLPU泪液中发现）显著（P < 0.0001）增强了对PMN的趋化活性。我们的数据突出了这些炎症介质在眼部炎症反应期间白细胞募集和活化中的可能病理生理作用。结果表明，GM-CSF、IL-8和LTB4在角膜病变期间具有活性，并且LTB4或IL-8可能在体内维持隐形眼镜诱发的PMN反应。
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