噬菌体φ29 pRNA的RNA间相互作用形成六聚体复合物用于病毒DNA转运。

Inter-RNA interaction of phage phi29 pRNA to form a hexameric complex for viral DNA transportation.

作者信息

Guo P, Zhang C, Chen C, Garver K, Trottier M

机构信息

Department of Pathobiology, Purdue Biochemistry and Molecular Biology Program, Purdue University, West Lafayette, Indiana 47907, USA.

出版信息

Mol Cell. 1998 Jul;2(1):149-55. doi: 10.1016/s1097-2765(00)80124-0.

DOI:10.1016/s1097-2765(00)80124-0

PMID:9702202

Abstract

Ds-DNA viruses package their DNA into a preformed protein shell (procapsid) during maturation. Bacteriophage phi29 requires an RNA (pRNA) to package its genomic DNA into the procapsid. We report here that the pRNA upper and lower loops are involved in RNA/RNA interactions. Mutation in only one loop results in inactive pRNAs. However, mixing of two, three and six inactive mutant pRNAs restores DNA packaging activity as long as an interlocking hexameric ring can be predicted to form by base pairing of the mutated loops in separate RNA molecules. The stoichiometry of pRNA for the packaging of one viral DNA genome is six. Homogeneous pRNA purified from a single band in denaturing gels showed six bands when rerun in native gels. These results suggest that six pRNAs form a hexameric ring by the intermolecular interaction of two RNA loops to serve as part of the DNA transportation machinery.

摘要

双链DNA病毒在成熟过程中将其DNA包装到预先形成的蛋白质外壳（原衣壳）中。噬菌体phi29需要一种RNA（pRNA）将其基因组DNA包装到原衣壳中。我们在此报告，pRNA的上下环参与RNA/RNA相互作用。仅一个环发生突变会导致pRNA无活性。然而，只要通过单独RNA分子中突变环的碱基配对可预测形成互锁的六聚体环，将两个、三个和六个无活性突变pRNA混合就能恢复DNA包装活性。包装一个病毒DNA基因组所需pRNA的化学计量比是六个。从变性凝胶中的单一条带纯化得到的均一pRNA在非变性凝胶中重新运行时显示出六个条带。这些结果表明，六个pRNA通过两个RNA环的分子间相互作用形成六聚体环，作为DNA运输机制的一部分。